|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Linker For Activation of T Cells (LAT) (pTyr191) antibody
|Synonyms||LAT1, pp36, MGC108666, LAT, MGC157208, lat|
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
|2 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: LAT, Linker for activation of T-cells family member 1, 36 kDa phospho-tyrosine adapter protein,pp36,p36-38|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic phospho-peptide corresponding to residues surrounding Tyr191 of human LAT was used as immunogen. The antibody only detects LAT phosphorylated on Tyrosine 191.|
|Molecular Weight||36/38 kDA|
Background: LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).
|Application Notes||The suggested dilution is: WB: ~~ 1:500 IP: ~~ 1:10 ICC: ~~ 1:50 IHC: ~~ 1:50|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||LAT Antibody Phospho (pY191) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
Zhang, Sloan-Lancaster, Kitchen et al.: "LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation." in: Cell, Vol. 92, Issue 1, pp. 83-92, 1998 (PubMed).
Weber, Orstavik, Torgersen et al.: "Molecular cloning of the cDNA encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by T cells and natural killer cells." in: The Journal of experimental medicine, Vol. 187, Issue 7, pp. 1157-61, 1998 (PubMed).