Linker For Activation of T Cells (LAT) (pTyr191) antibody

Antigen: Linker For Activation of T Cells (LAT)

Synonyms: LAT1, pp36, MGC108666, LAT, MGC157208, lat

Binding Site: pTyr191

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649977

Additional Information

Alternative name: LAT

Gene ID: 27040

UniProt: O43561

Description: Other names: LAT, Linker for activation of T-cells family member 1, 36 kDa phospho-tyrosine adapter protein,pp36,p36-38

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic phospho-peptide corresponding to residues surrounding Tyr191 of human LAT was used as immunogen. The antibody only detects LAT phosphorylated on Tyrosine 191.

Molecular Weight: 36/38 kDA

Comments:

Background: LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500 IP: ~~ 1:10 ICC: ~~ 1:50 IHC: ~~ 1:50

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: LAT Antibody Phospho (pY191) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Zhang, Sloan-Lancaster, Kitchen et al.: "LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation." in: Cell, Vol. 92, Issue 1, pp. 83-92, 1998 (PubMed).

Weber, Orstavik, Torgersen et al.: "Molecular cloning of the cDNA encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by T cells and natural killer cells." in: The Journal of experimental medicine, Vol. 187, Issue 7, pp. 1157-61, 1998 (PubMed).