LCK/p56 antibody

Antigen: LCK/p56

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649981

Additional Information

Gene ID: 3932

UniProt: P06239

Description: Other names: LCK, Proto-oncogene tyrosine-protein kinase LCK, Lymphocyte cell-specific protein-tyrosine kinase,p56-LCK,LSK,T cell-specific protein-tyrosine kinase

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues before SH3 domain of human Lck was used as immunogen. The antibody does not cross-react with other SRC family member.

Molecular Weight: 56 kDA

Comments:

Background: The human T-cell or lymphocyte specific gene Lck/p56 is a member of the Src family of non-receptors tyrosine kinase. Mostly expressed in T cells, Lck has been shown to be critical for the normal development of T lymphocytes (1). Additionally, the N-terminal region of the tyrosine kinase Lck interacts with the cytoplasmic domains of CD4 and CD8 (2). Autophosphorylation at Tyr-394 appears to be required for maximum catalytic activity (3) but it can also be suppressed by phosphorylation of the carboxyl-terminal tyrosine Tyr-505 (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 FC: ~~ 1:20 ICC: ~~ 1:100 IHC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: LCK/p56 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Chromatin

Restrictions: For Research Use only

Publications

Product: Rudd, Trevillyan, Dasgupta et al.: "The CD4 receptor is complexed in detergent lysates to a protein-tyrosine kinase (pp58) from human T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 14, pp. 5190-4, 1988 (PubMed).

Nakamura, Hori, Sato et al.: "Redox regulation of a src family protein tyrosine kinase p56lck in T cells." in: Oncogene, Vol. 8, Issue 11, pp. 3133-9, 1993 (PubMed).