LCK/p56 antibody

Details for Product No. ABIN649981
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Antigen
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Application
Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 2 references available
Quantity 100 μL
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Catalog No. ABIN649981
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Specificity A synthetic peptide corresponding to residues before SH3 domain of human Lck was used as immunogen. The antibody does not cross-react with other SRC family member.
Background The human T-cell or lymphocyte specific gene Lck/p56 is a member of the Src family of non-receptors tyrosine kinase. Mostly expressed in T cells, Lck has been shown to be critical for the normal development of T lymphocytes (1). Additionally, the N-terminal region of the tyrosine kinase Lck interacts with the cytoplasmic domains of CD4 and CD8 (2). Autophosphorylation at Tyr-394 appears to be required for maximum catalytic activity (3) but it can also be suppressed by phosphorylation of the carboxyl-terminal tyrosine Tyr-505 (4).
Synonyms: LCK, Proto-oncogene tyrosine-protein kinase LCK, Lymphocyte cell-specific protein-tyrosine kinase,p56-LCK,LSK,T cell-specific protein-tyrosine kinase
Molecular Weight 56 kDA
Gene ID 3932
UniProt P06239
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Chromatin
Application Notes IHC: = 1:250-500, WB: = 1:1000-10000
Comment

Background: The human T-cell or lymphocyte specific gene Lck/p56 is a member of the Src family of non-receptors tyrosine kinase. Mostly expressed in T cells, Lck has been shown to be critical for the normal development of T lymphocytes (1). Additionally, the N-terminal region of the tyrosine kinase Lck interacts with the cytoplasmic domains of CD4 and CD8 (2). Autophosphorylation at Tyr-394 appears to be required for maximum catalytic activity (3) but it can also be suppressed by phosphorylation of the carboxyl-terminal tyrosine Tyr-505 (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment LCK/p56 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Rudd, Trevillyan, Dasgupta et al.: "The CD4 receptor is complexed in detergent lysates to a protein-tyrosine kinase (pp58) from human T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 14, pp. 5190-4, 1988 (PubMed).

Nakamura, Hori, Sato et al.: "Redox regulation of a src family protein tyrosine kinase p56lck in T cells." in: Oncogene, Vol. 8, Issue 11, pp. 3133-9, 1993 (PubMed).

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