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Details for Product No. ABIN649982

LDH-A (Muscle Subunit) antibody

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Antigen
Reactivity
Human, Rat (Rattus)
Host
Rabbit
Clonality Monoclonal
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)
Pubmed 1 reference available
Catalog no. ABIN649982
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic peptide corresponding to residues in human LDH-A (muscle subunit) was used as an immunogen.
Background Lactate dehydrogenase (LDH) is a ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). Lactate dehydrogenase A (LDH-A, LDH muscle subunit, LDH-M) is involved in the final step of anaerobic glycoysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that cause deficiency in LDH-A has been implicated in exertional myoglobinuria (3).
Synonyms: LDHA, L-lactate dehydrogenase A chain, Renal carcinoma antigen NY-REN-59,Cell proliferation-inducing gene 19 protein
Molecular Weight 36 kDA
Gene ID 3939
UniProt P00338
Research Area Signaling, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:1000~5000 FC: ~~ 1:50 ICC: ~~ 1:100~250 IHC: ~~ 1:250~500
Comment

Background: Lactate dehydrogenase (LDH) is a ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). Lactate dehydrogenase A (LDH-A, LDH muscle subunit, LDH-M) is involved in the final step of anaerobic glycoysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that cause deficiency in LDH-A has been implicated in exertional myoglobinuria (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment LDH-A Antibody (muscle subunit) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Kanno, Sudo, Maekawa et al.: "Lactate dehydrogenase M-subunit deficiency: a new type of hereditary exertional myopathy." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 173, Issue 1, pp. 89-98, 1988 (PubMed).

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