LIM Domain Kinase 2 (LIMK2) (C-Term) antibody

Antigen: LIM Domain Kinase 2 (LIMK2)

Synonyms: C85310, Limk2a, Limk2b, 9430059K01, A930024P04Rik, Link2, LIMK, xlimk2, zgc:91990, wu:fa97c09, wu:fk73e11, LIMK2, MGC134164

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649993

Additional Information

Alternative name: LIMK2

Gene ID: 3985

UniProt: P53671

Description: Other names: LIMK1, LIMK, LIM domain kinase 1

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the C-terminus of human LIMK2 was used as an immunogen.

Molecular Weight: 70 kDA

Comments:

Background: LIM-kinase 2 (LIMK2), a 75-kDa member of the LIM kinase family, is responsible for regulating actin polymerization via phosphorylation and inactivation of the actin depolymerizing factors ADF/cofilin (1). LIMK2 protein is found in the cytoplasm during interphase, redistributed to the mitotic spindle and then to the spindle midzone during anaphase to telophase (2). It has been shown that ROCK, a downstream protein kinase of Rho, activates LIMK2 by directly phosphorylates threonine 505 residue. Since activity of LIMK2 is cell cycle dependant, LIMK2 activity is markedly increased when HeLa cells are synchronized at mitosis with nocodazole treatment (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500 IP: ~~ 1:50 FC: ~~ 1:10 ICC: ~~ 1:50~100 IHC: ~~ 1:50~100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: LIMK2 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Sumi, Matsumoto, Nakamura: "Specific activation of LIM kinase 2 via phosphorylation of threonine 505 by ROCK, a Rho-dependent protein kinase." in: The Journal of biological chemistry, Vol. 276, Issue 1, pp. 670-6, 2001 (PubMed).