Lipin 1 (LPIN1) antibody

Details for Product No. ABIN649995
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms LPIN1, pap1, PAP1, 4631420P06, Kiaa0188, Lipin1, fld, mKIAA0188, zgc:194552, zgc:194558, lipin1
Reactivity
Human
(56), (44), (27), (14), (14), (12), (1), (1)
Host
Rabbit
(62), (5), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(38), (29), (16), (10), (7), (3), (2), (2), (1)
Pubmed 3 references available
Quantity 100 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN649995
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic peptide corresponding to residues in human Lipin-1 was used as an immunogen.
Alternative Name Lipin-1
Background Lipin-1 is a Mg(2)+-dependent phosphatidate phosphatase (PAP) enzyme which catalyzes the conversion of phosphatidate to diacylglycerol, a key reaction in glycerolipid biosynthesis (1). It plays critical roles in controlling energy metabolism, has establishing the adipogenic gene expression program during early stages of differentiation, and in triglyceride accumulation in mature adipocytes (2, 3). Lipin-1 also functions as a transcriptional coactivator in conjunction with members of the peroxisome proliferator-activated receptor (PPAR) family (2). There are two isoforms of Lipin-1 (alpha and beta) which appear to serve distinct functions in adipocytes, with unique expression dynamics, subcellular localization, and effects on gene expression. Lipin-1B is the predominant form in mature adipocytes. Lipin-1 expression levels are associated with insulin sensitivity, metabolic rate, hypertension, and risk for the metabolic syndrome (4).
Molecular Weight 140 kDA
Gene ID 51286
UniProt Q14693
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure, Transcription Factors, Signaling, Metabolism
Application Notes The suggested dilution is: WB: = 1:20000 , IHC: = 1:50-100
Comment

Background: Lipin-1 is a Mg(2)+-dependent phosphatidate phosphatase (PAP) enzyme which catalyzes the conversion of phosphatidate to diacylglycerol, a key reaction in glycerolipid biosynthesis (1). It plays critical roles in controlling energy metabolism, has establishing the adipogenic gene expression program during early stages of differentiation, and in triglyceride accumulation in mature adipocytes (2, 3). Lipin-1 also functions as a transcriptional coactivator in conjunction with members of the peroxisome proliferator-activated receptor (PPAR) family (2). There are two isoforms of Lipin-1 (alpha and beta) which appear to serve distinct functions in adipocytes, with unique expression dynamics, subcellular localization, and effects on gene expression. Lipin-1B is the predominant form in mature adipocytes. Lipin-1 expression levels are associated with insulin sensitivity, metabolic rate, hypertension, and risk for the metabolic syndrome (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Lipin-1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Croce, Eagon, LaRiviere et al.: "Hepatic lipin 1beta expression is diminished in insulin-resistant obese subjects and is reactivated by marked weight loss." in: Diabetes, Vol. 56, Issue 9, pp. 2395-9, 2007 (PubMed).

Reue, Zhang: "The lipin protein family: dual roles in lipid biosynthesis and gene expression." in: FEBS letters, Vol. 582, Issue 1, pp. 90-6, 2007 (PubMed).

Zhang, OLoughlin, Brindley et al.: "Regulation of lipin-1 gene expression by glucocorticoids during adipogenesis." in: Journal of lipid research, Vol. 49, Issue 7, pp. 1519-28, 2008 (PubMed).

Validation Images
Did you look for something else?
back to top