Lipin 1 (LPIN1) antibody

Antigen: Lipin 1 (LPIN1)

Synonyms: PAP1, KIAA0188, DKFZp781P1796, fld, Lipin1, Kiaa0188, mKIAA0188, 4631420P06, LPIN1, MGC146217

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649995

Additional Information

Alternative name: Lipin-1

Gene ID: 51286

UniProt: Q14693

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human Lipin-1 was used as an immunogen.

Molecular Weight: 140 kDA

Comments:

Background: Lipin-1 is a Mg(2)+-dependent phosphatidate phosphatase (PAP) enzyme which catalyzes the conversion of phosphatidate to diacylglycerol, a key reaction in glycerolipid biosynthesis (1). It plays critical roles in controlling energy metabolism, has establishing the adipogenic gene expression program during early stages of differentiation, and in triglyceride accumulation in mature adipocytes (2, 3). Lipin-1 also functions as a transcriptional coactivator in conjunction with members of the peroxisome proliferator-activated receptor (PPAR) family (2). There are two isoforms of Lipin-1 (alpha and beta) which appear to serve distinct functions in adipocytes, with unique expression dynamics, subcellular localization, and effects on gene expression. Lipin-1B is the predominant form in mature adipocytes. Lipin-1 expression levels are associated with insulin sensitivity, metabolic rate, hypertension, and risk for the metabolic syndrome (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:20000 IP: ~~ 1:40 FC: ~~ 1:10 ICC: ~~ 1:100~250 IHC: ~~ 1:50~100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Lipin-1 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure, Transcription Factors, Signaling, Metabolism

Restrictions: For Research Use only

Publications

Product: Croce, Eagon, LaRiviere et al.: "Hepatic lipin 1beta expression is diminished in insulin-resistant obese subjects and is reactivated by marked weight loss." in: Diabetes, Vol. 56, Issue 9, pp. 2395-9, 2007 (PubMed).

Reue, Zhang: "The lipin protein family: dual roles in lipid biosynthesis and gene expression." in: FEBS letters, Vol. 582, Issue 1, pp. 90-6, 2007 (PubMed).

Zhang, OLoughlin, Brindley et al.: "Regulation of lipin-1 gene expression by glucocorticoids during adipogenesis." in: Journal of lipid research, Vol. 49, Issue 7, pp. 1519-28, 2008 (PubMed).