MAP-2 (pSer136) antibody

Antigen: MAP-2

Binding Site: pSer136

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650006

Additional Information

Alternative name: MAP2

Gene ID: 4133

UniProt: P11137

Characteristics: Rabbit Monoclonal Antibody

Specificity: A phospho-specific peptide corresponding to residues surrounding Serine 136 of human MAP2 was used as an immunogen.

Molecular Weight: 280/75 kDA

Comments:

Background: Microtubule-associated protein-2 (MAP-2) is a family of heat-stable, phosphoproteins expressed predominantly in the cell body and dendrites of neurons. Three major MAP-2 isoforms, (MAP2a, and MAP2b (280 kDa), MAP2c (75 kDa)) are differentially expressed during the development of the nervous system and have an important role in microtubule dynamics (1). MAP2 is proposed to play important roles in the outgrowth of neuronal processes, synaptic plasticity and neuronal cell death. MAP2 may perform many functions including the nucleation and stabilization of microtubules, the regulation of organelle transport within axons and dendrites, as well as the anchorage of regulatory proteins such as protein kinases which may be important for signal transduction (2). MAP2 is an important cytoskeleton protein whose phosphorylation in response to extracellular signal is involved in the regulation of neurite outgrowth and neuronal plasticity. Findings suggest that epinephrine induces phosphorylation of MAP2c at Serine 136 through an alpha 2-adrenoceptor mediated, ERK/PKC-dependent signaling pathway, which may contribute to the stabilization of neuritis (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:10~100 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: MAP2 Antibody Phospho (pS136) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Shafit-Zagardo, Kalcheva: "Making sense of the multiple MAP-2 transcripts and their role in the neuron." in: Molecular neurobiology, Vol. 16, Issue 2, pp. 149-62, 1999 (PubMed).

Sánchez, Díaz-Nido, Avila: "Phosphorylation of microtubule-associated protein 2 (MAP2) and its relevance for the regulation of the neuronal cytoskeleton function." in: Progress in neurobiology, Vol. 61, Issue 2, pp. 133-68, 2000 (PubMed).

Liu, Saad, Shen et al.: "Diagnostic value of microtubule-associated protein-2 (MAP-2) for neuroendocrine neoplasms." in: Advances in anatomic pathology, Vol. 10, Issue 2, pp. 101-6, 2003 (PubMed).