MAPILC3A (C-Term) antibody

Details for Product No. ABIN650008
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Antigen
Epitope
C-Term
(1)
Reactivity
Human, Rat (Rattus)
(2), (2)
Host
Rabbit
(2)
Clonality
Monoclonal
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(2), (1)
Pubmed 2 references available
Quantity 100 µL
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Catalog No. ABIN650008
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Specificity A synthetic peptide corresponding to residues on the C-terminus of human MAPILC3A was used as an immunogen.
Background Microtubule-associated MAPILC3A constitute nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. MAPILC3A is composed of a heavy chain and multiple light-chain subunits. Amino acid sequencing of a 120 kd microtubule-binding and light chain-binding fragment of the heavy chain reveals that light chain 1 binds near the heavy chain N-terminus. These data indicate that the heavy chain and light chain 1 are produced by proteolytic processing of a MAP1B polyprotein and form a complex microtubule-binding domain (1). There are three human orthologs of the rat Map1LC3, named MAP1LC3A, MAP1LC3B, and MAP1LC3C. The three isoforms of human MAP1LC3 exhibit distinct expression patterns in different human tissues and also differ in their post-translation modifications. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form (2).
Synonyms: MAP1LC3A, Microtubule-associated proteins 1A/1B light chain 3A, Microtubule-associated protein 1 light chain 3 alpha,MAP1A/1B light chain 3 A,MAP1A/MAP1B LC3 A,MAP1 light chain 3-like protein 1,Autophagy-related protein LC3 A,Autophagy-related ubiquitin-l
Molecular Weight 16 kDA
Gene ID 84557
UniProt Q9H492
Research Area Cell Structure, Autophagy
Application Notes IHC: = 1:100-250, WB: = 1:10000
Comment

Background: Microtubule-associated MAPILC3A constitute nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. MAPILC3A is composed of a heavy chain and multiple light-chain subunits. Amino acid sequencing of a 120 kd microtubule-binding and light chain-binding fragment of the heavy chain reveals that light chain 1 binds near the heavy chain N-terminus. These data indicate that the heavy chain and light chain 1 are produced by proteolytic processing of a MAP1B polyprotein and form a complex microtubule-binding domain (1). There are three human orthologs of the rat Map1LC3, named MAP1LC3A, MAP1LC3B, and MAP1LC3C. The three isoforms of human MAP1LC3 exhibit distinct expression patterns in different human tissues and also differ in their post-translation modifications. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form (2).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment MAPILC3A Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Hammarback, Obar, Hughes et al.: "MAP1B is encoded as a polyprotein that is processed to form a complex N-terminal microtubule-binding domain." in: Neuron, Vol. 7, Issue 1, pp. 129-39, 1991 (PubMed).

He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

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