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Details for Product No. ABIN650012

MAPK-p44 (N-Term) antibody

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Antigen
Epitope
N-Term
Reactivity
Human
Host
Rabbit
Clonality Monoclonal
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)
Pubmed 3 references available
Catalog no. ABIN650012
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic peptide corresponding to residues near N-terminus of human ERK1 was used as immunogen. The antibody does not cross-react with other MAP kinases.
Background P44 MAP Kinase (Erk1) is a member of a family of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth and differentiation (1,2). Erk1 regulates meiosis and mitosis by phosphorylating a number of transcription factors such as ELK-1. Activation of Erk1 occurs through phosphorylation of threonine 202 and tyrosine 204 (of human MAP kinase) (3). Erk1 is also known to autophosphorylate on tyrosine (3).
Synonyms: MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 3, p44-ERK1,ERT2,p44-MAPK,Microtubule-associated protein 2 kinase
Molecular Weight 44 kDA
Gene ID 5595
UniProt P27361
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:10000 IP: ~~ 1:80 FC: ~~ 1:40 ICC: ~~ 1:100 IHC: ~~ 1:100
Comment

Background: p44 MAP Kinase (Erk1) is a member of a family of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth and differentiation (1,2). Erk1 regulates meiosis and mitosis by phosphorylating a number of transcription factors such as ELK-1. Activation of Erk1 occurs through phosphorylation of threonine 202 and tyrosine 204 (of human MAP kinase) (3). Erk1 is also known to autophosphorylate on tyrosine (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment MAPK-p44 Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Payne, Rossomando, Martino et al.: "Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase)." in: The EMBO journal, Vol. 10, Issue 4, pp. 885-92, 1991 (PubMed).

Charest, Mordret, Harder et al.: "Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4679-90, 1993 (PubMed).

Marshall: "Specificity of receptor tyrosine kinase signaling: transient versus sustained extracellular signal-regulated kinase activation." in: Cell, Vol. 80, Issue 2, pp. 179-85, 1995 (PubMed).

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