MAPK-p44 (N-Term) antibody

Antigen: MAPK-p44

Binding Site: N-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN650012

Additional Information

Gene ID: 5595

UniProt: P27361

Description: Other names: MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 3, p44-ERK1,ERT2,p44-MAPK,Microtubule-associated protein 2 kinase

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near N-terminus of human ERK1 was used as immunogen. The antibody does not cross-react with other MAP kinases.

Molecular Weight: 44 kDA

Comments:

Background: p44 MAP Kinase (Erk1) is a member of a family of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth and differentiation (1,2). Erk1 regulates meiosis and mitosis by phosphorylating a number of transcription factors such as ELK-1. Activation of Erk1 occurs through phosphorylation of threonine 202 and tyrosine 204 (of human MAP kinase) (3). Erk1 is also known to autophosphorylate on tyrosine (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:10000 IP: ~~ 1:80 FC: ~~ 1:40 ICC: ~~ 1:100 IHC: ~~ 1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: MAPK-p44 Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Payne, Rossomando, Martino et al.: "Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase)." in: The EMBO journal, Vol. 10, Issue 4, pp. 885-92, 1991 (PubMed).

Charest, Mordret, Harder et al.: "Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4679-90, 1993 (PubMed).

Marshall: "Specificity of receptor tyrosine kinase signaling: transient versus sustained extracellular signal-regulated kinase activation." in: Cell, Vol. 80, Issue 2, pp. 179-85, 1995 (PubMed).