|+1 404 474 4654|
|+1 888 205 9894 (TF)|
MAPK-p44 (N-Term) antibody
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)
|3 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 3, p44-ERK1,ERT2,p44-MAPK,Microtubule-associated protein 2 kinase|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues near N-terminus of human ERK1 was used as immunogen. The antibody does not cross-react with other MAP kinases.|
|Molecular Weight||44 kDA|
Background: p44 MAP Kinase (Erk1) is a member of a family of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth and differentiation (1,2). Erk1 regulates meiosis and mitosis by phosphorylating a number of transcription factors such as ELK-1. Activation of Erk1 occurs through phosphorylation of threonine 202 and tyrosine 204 (of human MAP kinase) (3). Erk1 is also known to autophosphorylate on tyrosine (3).
|Application Notes||The suggested dilution is: WB: ~~ 1:10000 IP: ~~ 1:80 FC: ~~ 1:40 ICC: ~~ 1:100 IHC: ~~ 1:100|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||MAPK-p44 Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Payne, Rossomando, Martino et al.: "Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase)." in: The EMBO journal, Vol. 10, Issue 4, pp. 885-92, 1991 (PubMed).
Charest, Mordret, Harder et al.: "Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4679-90, 1993 (PubMed).
Marshall: "Specificity of receptor tyrosine kinase signaling: transient versus sustained extracellular signal-regulated kinase activation." in: Cell, Vol. 80, Issue 2, pp. 179-85, 1995 (PubMed).