Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) antibody

Details for Product No. ABIN650013
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Antigen
Synonyms 80K-L, MACS, PKCSL, PRKCSL, Macs, KINC, macs, marcks, wu:fb55c06, wu:fb92d04, wu:fc19b04, wu:fc44a10, zgc:110235, MGC89127, MARCKS
Reactivity
Human
(199), (140), (129), (54), (40), (36), (25), (3), (3), (1), (1), (1)
Host
Rabbit
(198), (19), (3)
Clonality
Monoclonal
Conjugate
Un-conjugated
(6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(161), (68), (52), (40), (33), (24), (23), (8), (2), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN650013
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic peptide corresponding to residues on human MARCKS was used as an immunogen.
Alternative Name MARCKS
Background The expression of a major cellular substrate for protein kinase C, the MARCKS protein, is regulated in a cell-, tissue-, and developmental stage-specific fashion, in addition, this expression can be stimulated acutely by various cytokines in certain cell types (1). MARCKS) binds calmodulin and is a predominant, specific substrate of PKC which is phosphorylated during macrophage and neutrophil activation, growth factor-dependent mitogenesis and neurosecretion, it is redistributed from plasma membrane to cytoplasm when phosphorylated and is involved in leukocyte motility. It has been reported that MARCKS is a filamentous (F) actin crosslinking protein, with activity that is inhibited by PKC-mediated phosphorylation and by binding to calcium-calmodulin. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and PKC signal transduction pathways in the regulation of the actin cytoskeleton (2).
Synonyms: MARCKS, MACS, PRKCSL, Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate, 80 kDa protein, light chain
Molecular Weight 87 kDA
Gene ID 4082
UniProt P29966
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes IHC: = 1:100-250, WB: = 1:1000-10000
Comment

Background: The expression of a major cellular substrate for protein kinase C, the MARCKS protein, is regulated in a cell-, tissue-, and developmental stage-specific fashion, in addition, this expression can be stimulated acutely by various cytokines in certain cell types (1). MARCKS) binds calmodulin and is a predominant, specific substrate of PKC which is phosphorylated during macrophage and neutrophil activation, growth factor-dependent mitogenesis and neurosecretion, it is redistributed from plasma membrane to cytoplasm when phosphorylated and is involved in leukocyte motility. It has been reported that MARCKS is a filamentous (F) actin crosslinking protein, with activity that is inhibited by PKC-mediated phosphorylation and by binding to calcium-calmodulin. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and PKC signal transduction pathways in the regulation of the actin cytoskeleton (2).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment MARCKS Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Hartwig, Thelen, Rosen et al.: "MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin." in: Nature, Vol. 356, Issue 6370, pp. 618-22, 1992 (PubMed).

Harlan, Graff, Stumpo et al.: "The human myristoylated alanine-rich C kinase substrate (MARCKS) gene (MACS). Analysis of its gene product, promoter, and chromosomal localization." in: The Journal of biological chemistry, Vol. 266, Issue 22, pp. 14399-405, 1991 (PubMed).

Hosts (198), (19), (3)
Reactivities (199), (140), (129), (54), (40), (36), (25), (3), (3), (1), (1), (1)
Applications (161), (68), (52), (40), (33), (24), (23), (8), (2), (1), (1), (1)
Conjugates (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Epitopes (26), (26), (25), (25), (24), (13), (7), (6), (6), (5), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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