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Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) antibody
|Synonyms||MACS, 80K-L, PKCSL, PRKCSL, FLJ14368, FLJ90045, KINC, Macs, marcks, MGC89127, MARCKS, MGC137530|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)
|2 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: MARCKS, MACS, PRKCSL, Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate, 80 kDa protein, light chain|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues on human MARCKS was used as an immunogen.|
|Molecular Weight||87 kDA|
Background: The expression of a major cellular substrate for protein kinase C, the MARCKS protein, is regulated in a cell-, tissue-, and developmental stage-specific fashion, in addition, this expression can be stimulated acutely by various cytokines in certain cell types (1). MARCKS) binds calmodulin and is a predominant, specific substrate of PKC which is phosphorylated during macrophage and neutrophil activation, growth factor-dependent mitogenesis and neurosecretion, it is redistributed from plasma membrane to cytoplasm when phosphorylated and is involved in leukocyte motility. It has been reported that MARCKS is a filamentous (F) actin crosslinking protein, with activity that is inhibited by PKC-mediated phosphorylation and by binding to calcium-calmodulin. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and PKC signal transduction pathways in the regulation of the actin cytoskeleton (2).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000~10000 FC: ~~ 1:100 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||MARCKS Antibody can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Phospho-specific antibodies, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
Hartwig, Thelen, Rosen et al.: "MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin." in: Nature, Vol. 356, Issue 6370, pp. 618-22, 1992 (PubMed).
Harlan, Graff, Stumpo et al.: "The human myristoylated alanine-rich C kinase substrate (MARCKS) gene (MACS). Analysis of its gene product, promoter, and chromosomal localization." in: The Journal of biological chemistry, Vol. 266, Issue 22, pp. 14399-405, 1991 (PubMed).