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Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer159, pSer163) antibody
|Synonyms||MACS, 80K-L, PKCSL, PRKCSL, FLJ14368, FLJ90045, KINC, Macs, marcks, MGC89127, MARCKS, MGC137530|
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunoprecipitation (IP)
|2 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: MARCKS, MACS, PRKCSL, Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate, 80 kDa protein, light chain|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic phospho-peptide corresponding to residues surrounding serine 159 and 163 of human MARCKS protein. The orthologous phosphorylation sites in mouse and rat is serine 152 and 156|
|Molecular Weight||87 kDA|
Background: The expression of a major cellular substrate for protein kinase C, the MARCKS protein, is regulated in a cell-, tissue-, and developmental stage-specific fashion, in addition, this expression can be stimulated acutely by various cytokines in certain cell types (1). As a filamentous (F) actin crosslinking protein, MARCKS binds to actin, calmodulin, and synapsin. In murine, MARCKS binding activity is regulated by PKC phosphorylation at Ser152, 156, and 163 of MARCKS during macrophage and neutrophil activation, growth factor-dependent mitogenesis and neurosecretion. After phosphorylation, MARCKS is redistributed from plasma membrane to cytoplasm and is involved in leukocyte motility. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and PKC signal transduction pathways in the regulation of the actin cytoskeleton (2).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000~2000 IP: ~~ 1:40|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||MARCKS Antibody Phospho (pS159/S163) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Phospho-specific antibodies, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
Hartwig, Thelen, Rosen et al.: "MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin." in: Nature, Vol. 356, Issue 6370, pp. 618-22, 1992 (PubMed).
Harlan, Graff, Stumpo et al.: "The human myristoylated alanine-rich C kinase substrate (MARCKS) gene (MACS). Analysis of its gene product, promoter, and chromosomal localization." in: The Journal of biological chemistry, Vol. 266, Issue 22, pp. 14399-405, 1991 (PubMed).