Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer159), (pSer163) antibody

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Antigen
Synonyms marcks, MGC89127, MARCKS, Macs, PKCSL, KINC, macs, wu:fb55c06, wu:fb92d04, wu:fc19b04, wu:fc44a10, zgc:110235, 80K-L, MACS, PRKCSL
Epitope
pSer159, pSer163
(29), (28), (26), (26), (25), (15), (9), (7), (6), (6), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(208), (141), (131), (54), (40), (36), (25), (3), (3), (1), (1), (1)
Host
Rabbit
(210), (17), (3)
Clonality
Monoclonal
Conjugate
Un-conjugated
(6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application
Western Blotting (WB)
(170), (61), (58), (40), (29), (29), (12), (5), (4), (3), (2), (1), (1)
Pubmed 2 references available
Quantity 100 μL
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Catalog No. ABIN650014
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Specificity A synthetic phospho-peptide corresponding to residues surrounding serine 159 and 163 of human MARCKS protein. The orthologous phosphorylation sites in mouse and rat is serine 152 and 156
Alternative Name MARCKS
Background The expression of a major cellular substrate for protein kinase C, the MARCKS protein, is regulated in a cell-, tissue-, and developmental stage-specific fashion, in addition, this expression can be stimulated acutely by various cytokines in certain cell types (1). As a filamentous (F) actin crosslinking protein, MARCKS binds to actin, calmodulin, and synapsin. In murine, MARCKS binding activity is regulated by PKC phosphorylation at Ser152, 156, and 163 of MARCKS during macrophage and neutrophil activation, growth factor-dependent mitogenesis and neurosecretion. After phosphorylation, MARCKS is redistributed from plasma membrane to cytoplasm and is involved in leukocyte motility. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and PKC signal transduction pathways in the regulation of the actin cytoskeleton (2).
Synonyms: MARCKS, MACS, PRKCSL, Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate, 80 kDa protein, light chain
Molecular Weight 87 kDA
Gene ID 4082
UniProt P29966
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-2000
Comment

Background: The expression of a major cellular substrate for protein kinase C, the MARCKS protein, is regulated in a cell-, tissue-, and developmental stage-specific fashion, in addition, this expression can be stimulated acutely by various cytokines in certain cell types (1). As a filamentous (F) actin crosslinking protein, MARCKS binds to actin, calmodulin, and synapsin. In murine, MARCKS binding activity is regulated by PKC phosphorylation at Ser152, 156, and 163 of MARCKS during macrophage and neutrophil activation, growth factor-dependent mitogenesis and neurosecretion. After phosphorylation, MARCKS is redistributed from plasma membrane to cytoplasm and is involved in leukocyte motility. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and PKC signal transduction pathways in the regulation of the actin cytoskeleton (2).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment MARCKS Antibody Phospho (pS159/S163) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Hartwig, Thelen, Rosen et al.: "MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin." in: Nature, Vol. 356, Issue 6370, pp. 618-22, 1992 (PubMed).

Harlan, Graff, Stumpo et al.: "The human myristoylated alanine-rich C kinase substrate (MARCKS) gene (MACS). Analysis of its gene product, promoter, and chromosomal localization." in: The Journal of biological chemistry, Vol. 266, Issue 22, pp. 14399-405, 1991 (PubMed).

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