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Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer159, pSer163) antibody

Antigen

Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS)
Synonyms MACS, 80K-L, PKCSL, PRKCSL, FLJ14368, FLJ90045, KINC, Macs, marcks, MGC89127, MARCKS, MGC137530
Binding Site
Alternatives

pSer159, pSer163
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunoprecipitation (IP)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN650014
Quantity 100ul
Price 507.14 $   Plus shipping costs $45.00
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Additional Information
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Alternative name MARCKS
Gene ID 4082
UniProt P29966
Description Other names: MARCKS, MACS, PRKCSL, Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate, 80 kDa protein, light chain
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic phospho-peptide corresponding to residues surrounding serine 159 and 163 of human MARCKS protein. The orthologous phosphorylation sites in mouse and rat is serine 152 and 156
Molecular Weight 87 kDA
Comments

Background: The expression of a major cellular substrate for protein kinase C, the MARCKS protein, is regulated in a cell-, tissue-, and developmental stage-specific fashion, in addition, this expression can be stimulated acutely by various cytokines in certain cell types (1). As a filamentous (F) actin crosslinking protein, MARCKS binds to actin, calmodulin, and synapsin. In murine, MARCKS binding activity is regulated by PKC phosphorylation at Ser152, 156, and 163 of MARCKS during macrophage and neutrophil activation, growth factor-dependent mitogenesis and neurosecretion. After phosphorylation, MARCKS is redistributed from plasma membrane to cytoplasm and is involved in leukocyte motility. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and PKC signal transduction pathways in the regulation of the actin cytoskeleton (2).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:1000~2000 IP: ~~ 1:40
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage MARCKS Antibody Phospho (pS159/S163) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Hartwig, Thelen, Rosen et al.: "MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin." in: Nature, Vol. 356, Issue 6370, pp. 618-22, 1992 (PubMed).

Harlan, Graff, Stumpo et al.: "The human myristoylated alanine-rich C kinase substrate (MARCKS) gene (MACS). Analysis of its gene product, promoter, and chromosomal localization." in: The Journal of biological chemistry, Vol. 266, Issue 22, pp. 14399-405, 1991 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS)", type "Antibodies"
Hosts Rabbit (174), Mouse (7), Goat (1), Rat (1)
Reactivities Human (166), Rat (Rattus) (131), Mouse (Murine) (127), Cow (Bovine) (77), Chicken (57), Dog (Canine) (55), Pig (Porcine) (38), Xenopus laevis (3), Zebrafish (Danio rerio) (2), Cat (Feline) (1), Rabbit (1)
Applications Western Blotting (WB) (104), Immunofluorescence (IF) (93), ELISA (48), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (23), Immunohistochemistry (IHC) (21), Immunocytochemistry (ICC) (13), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Immunoprecipitation (IP) (9), Immunoelectron Microscopy (IEM) (4), Flow Cytometry (FACS) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Biotin (4), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), FITC (4), Gold (4), HRP (4), PE (4), PE,Cy3 (4), PE,Cy5 (4), PE,Cy5.5 (4), PE,Cy7 (4)
Epitopes pSer162 (26), pSer170 (25), pSer152,pSer156 (17), pSer158 (10), C-Term (8), pSer152, pSer156 (5), pSer156,pSer152 (5), N-Term (3), Ser162 (3), pSer158,Internal Region (3), Internal Region (2), Ser158 (2), pSer163 (2), Phosphospecific (1), Ser152, Ser156 (1), pSer152 (1), pSer152, pSer153 (1), phospho (1)