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Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) antibody

Antigen

Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1)
Synonyms TM, EAT, MCL1L, MCL1S, Mcl-1, BCL2L3, MGC1839, MGC104264
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN650018
Quantity 100ul
Price 450.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Mcl-1
Gene ID 4170
UniProt Q07820
Description Other names: MCL1, BCL2L3, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-related protein EAT/mcl1,mcl1/EAT,Bcl-2-like protein 3
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues of human Mcl-1 was used as immunogen. The antibody does not cross-react with other Bcl-2 family members.
Molecular Weight 40 kDA
Comments

Background: Mcl-1 is an anti-apoptotic member of Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway (1). Mcl-1 localizes to the mitochondria (2), interacts with and antagonizes pro-apoptotic Bcl-2 family members (3), and inhibits apoptosis by a number of cytotoxic stimuli (4).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:40 ICC: ~~ 1:250~1:500 IHC: ~~ 1:50
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Mcl-1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Research Area Ubiquitin-related antibodies, Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Cell Structure, Cell Cycle
Restrictions For Research Use only

Publications
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Product Kozopas, Yang, Buchan et al.: "MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3516-20, 1993 (PubMed).

Zhou, Qian, Kozopas et al.: "Mcl-1, a Bcl-2 family member, delays the death of hematopoietic cells under a variety of apoptosis-inducing conditions." in: Blood, Vol. 89, Issue 2, pp. 630-43, 1997 (PubMed).

Alternatives
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Alternatives for antigen "Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1)", type "Antibodies"
Hosts Rabbit (112), Mouse (39), Goat (4)
Reactivities Human (151), Mouse (Murine) (66), Rat (Rattus) (56), Dog (Canine) (25), Cow (Bovine) (22), Horse (Equine) (18), Sheep (Ovine) (18), Cat (Feline) (7), Chicken (4), Monkey (1), Pig (Porcine) (1), Rabbit (1)
Applications Western Blotting (WB) (114), Immunofluorescence (IF) (55), ELISA (54), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (51), Immunoprecipitation (IP) (26), Immunohistochemistry (IHC) (21), Immunocytochemistry (ICC) (17), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunohistochemistry (Fixed) (IHC (fx)) (5), Flow Cytometry (FACS) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunoelectron Microscopy (IEM) (2)
Conjugates Biotin (5), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2)
Epitopes C-Term (27), pSer159,pThr163 (18), AA 121-139 (9), Internal Region (9), N-Term (6), BH3 Domain (3), AA 1-327 (2), Center (2), Isoform 1 (2), Internal Region,AA 216-245 (1), Isoform 1,C-Term (1), Middle Region (1), pSer159 (1)