Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (C-Term) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1)

Synonyms: MEK1, MKK1, MAPKK1, PRKMK1, Mek1, MEKK1, Prkmk1

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650026

Additional Information

Alternative name: MEK1

Gene ID: 5604

UniProt: Q02750

Description: Other names: MAP2K1, MEK1, PRKMK1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1,MAPK/ERK kinase 1

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in C-terminus of human MEK1 was used as immunogen. The antibody does not cross-react with other MAP kinase kinase family members.

Molecular Weight: 45 kDA

Comments:

Background: MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000 IP: ~~ 1:100 FC: ~~ 1:50 ICC: ~~ 1:250~1:500 IHC: ~~ 1:250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: MEK1 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).

Zheng, Guan: "Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues." in: The EMBO journal, Vol. 13, Issue 5, pp. 1123-31, 1994 (PubMed).

Alessi, Saito, Campbell et al.: "Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1." in: The EMBO journal, Vol. 13, Issue 7, pp. 1610-9, 1994 (PubMed).

Zheng, Guan: "Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2." in: The Journal of biological chemistry, Vol. 268, Issue 15, pp. 11435-9, 1993 (PubMed).