Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (C-Term) antibody

Details for Product No. ABIN650026
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Antigen
Synonyms
Mek1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, MEKK1, Prkmk1, mek-2, si:ch211-242m18.2, wu:fj56a12, wu:fj61b01, zgc:56557, ATMEK1, F20B18.180, F20B18_180, MAP kinase/ ERK kinase 1, MITOGEN ACTIVATED PROTEIN  ... show more
Mek1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, MEKK1, Prkmk1, mek-2, si:ch211-242m18.2, wu:fj56a12, wu:fj61b01, zgc:56557, ATMEK1, F20B18.180, F20B18_180, MAP kinase/ ERK kinase 1, MITOGEN ACTIVATED PROTEIN KINASE KINASE, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1, NMAPKK, MEK show less
Epitope
C-Term
(32), (27), (26), (25), (24), (21), (21), (20), (19), (12), (12), (11), (8), (7), (7), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(407), (222), (211), (72), (46), (40), (33), (8), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(377), (41), (2), (2), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(10), (10), (10), (6), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(328), (123), (103), (92), (60), (50), (21), (20), (14), (3), (2), (1)
Pubmed 4 references available
Quantity 100 μL
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Catalog No. ABIN650026
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Specificity A synthetic peptide corresponding to residues in C-terminus of human MEK1 was used as immunogen. The antibody does not cross-react with other MAP kinase kinase family members.
Alternative Name MEK1
Background MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).
Synonyms: MAP2K1, MEK1, PRKMK1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1,MAPK/ERK kinase 1
Molecular Weight 45 kDA
Gene ID 5604
UniProt Q02750
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000 , IHC: = 1:250
Comment

Background: MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment MEK1 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).

Zheng, Guan: "Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues." in: The EMBO journal, Vol. 13, Issue 5, pp. 1123-31, 1994 (PubMed).

Alessi, Saito, Campbell et al.: "Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1." in: The EMBO journal, Vol. 13, Issue 7, pp. 1610-9, 1994 (PubMed).

Zheng, Guan: "Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2." in: The Journal of biological chemistry, Vol. 268, Issue 15, pp. 11435-9, 1993 (PubMed).

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