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Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (N-Term) antibody

Antigen

Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1)
Synonyms MEK1, MKK1, MAPKK1, PRKMK1, Mek1, MEKK1, Prkmk1
Binding Site
Alternatives

N-Term
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
4 references available
Certificates ISO 9001:2008
Catalog no. ABIN650027
Quantity 100ul  (Variants)
Price 450.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name MEK1
Gene ID 5604
UniProt Q02750
Description Other names: MAP2K1, MEK1, PRKMK1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1,MAPK/ERK kinase 1
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues in the N-terminus of human MEK1 was used as an immunogen. The antibody does not cross-react with other MAP kinase kinase family member.
Molecular Weight 45 kDA
Comments

Background: MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:500 IP: ~~ 1:30 FC: ~~ 1:30 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage MEK1 Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Cell Structure
Restrictions For Research Use only

Publications
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Product Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).

Zheng, Guan: "Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues." in: The EMBO journal, Vol. 13, Issue 5, pp. 1123-31, 1994 (PubMed).

Alessi, Saito, Campbell et al.: "Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1." in: The EMBO journal, Vol. 13, Issue 7, pp. 1610-9, 1994 (PubMed).

Zheng, Guan: "Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2." in: The Journal of biological chemistry, Vol. 268, Issue 15, pp. 11435-9, 1993 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1)", type "Antibodies"
Hosts Rabbit (297), Mouse (35), Chicken (1)
Reactivities Human (321), Mouse (Murine) (199), Rat (Rattus) (193), Pig (Porcine) (90), Horse (Equine) (54), Dog (Canine) (42), Cow (Bovine) (40), Chicken (38), Xenopus laevis (5), Monkey (2)
Applications Western Blotting (WB) (207), Immunofluorescence (IF) (124), ELISA (93), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (74), Immunohistochemistry (IHC) (64), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (18), Immunoprecipitation (IP) (18), Dot Blot (Dot) (9), Immunocytochemistry (ICC) (8), Immunoelectron Microscopy (IEM) (6), Flow Cytometry (FACS) (5), Immunohistochemistry (Fixed) (IHC (fx)) (1)
Conjugates Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), Biotin (6), Cy3 (6), Cy5 (6), Cy5.5 (6), Cy7 (6), FITC (6), Gold (6), HRP (6), PE (6), PE,Cy3 (6), PE,Cy5 (6), PE,Cy5.5 (6), PE,Cy7 (6)
Epitopes pThr292 (29), pThr386 (27), C-Term (24), pSer298 (21), pThr286 (20), pSer385 (18), N-Term (10), pThr291 (10), pSer217 (9), pSer221 (8), Internal Region (7), pSer222 (7), pThr386,C-Term (6), C-Term,AA 353-367 (4), pSer218 (4), pSer218, pSer222 (4), pSer298,Internal Region (3), Ser217 (2), Ser218 (2), Ser221 (2), Ser297 (2), Thr291 (2), Thr385 (2), pSer297 (2), pThr385 (2), AA 1-12 (1), C-Term,AA 268-296 (1), Center (1), Internal Region,AA 205-234 (1), Ser218,Ser222 (1), Thr285 (1), Thr386 (1), pSer217, pSer221 (1), pSer218,pSer221 (1), pSer218,pSer222 (1), pSer222,pSer226 (1)