MEK1/MEK2 (pSer218, pSer222) antibody

Antigen: MEK1/MEK2

Binding Site: pSer218, pSer222

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650030

Additional Information

Gene ID: 5604

UniProt: Q02750

Description: Other names: MAP2K1, MEK1, PRKMK1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1,MAPK/ERK kinase 1

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic phospho-peptide corresponding to residues surrounding Ser218 and Ser222 of human MEK1 was used as immunogen. The antibody only detects MEK1 phosphorylated on Serine 218 and 222.

Molecular Weight: 45 kDA

Comments:

Background: MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1: 5000~50000 IP: ~~ 1:50 ICC: ~~ 1:50~100 IHC: ~~ 1:50~100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: MEK1/MEK2 Antibody Phospho (pS218/222) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).

Zheng, Guan: "Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues." in: The EMBO journal, Vol. 13, Issue 5, pp. 1123-31, 1994 (PubMed).

Alessi, Saito, Campbell et al.: "Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1." in: The EMBO journal, Vol. 13, Issue 7, pp. 1610-9, 1994 (PubMed).

Zheng, Guan: "Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2." in: The Journal of biological chemistry, Vol. 268, Issue 15, pp. 11435-9, 1993 (PubMed).