Mesothelin (MSLN) antibody

Details for Product No. ABIN650040
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Antigen
Synonyms MPF, SMRP, MSLN
Reactivity
Human
(80), (12), (6), (2), (1), (1)
Host
Rabbit
(55), (30), (7), (3)
Clonality
Monoclonal
Conjugate
Un-conjugated
(2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(51), (37), (24), (13), (10), (10), (7), (6), (6), (4), (4), (4), (4), (3), (2)
Pubmed 4 references available
Catalog no. ABIN650040
Quantity 100 µL
Price
392.86 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic peptide corresponding to residues near the C-terminus of human Mesothelin was used as an immunogen. This antibody detects full-length Mesothelin precursor (69 kDa) and C-terminal cleaved form of Mesothelin (40 kDa).
Alternative Name Mesothelin
Background Mesothelin is a tumor differentiation antigen that is present on the surface of normal mesothelial cells lining the pleura, peritoneum and pericardium. It is over expressed in many human tumors, including malignant mesothelioma and ovarian, pancreatic and lung adenocarcinomas (1). The normal biologic function of mesothelin is unknown but recent studies have shown that it forms a strong and specific complex with MUC16, a binding which has been suggested to be the basis of ovarian cancer metastasis (2). Initially, mesothelin is produced as a polypeptide with a hydrophobic tail which is removed and replaced by phosphatidylinositol. After glycosylation at the one or more of its four putative N-linked glycosylation sites, it is cleaved by a protease to yield the 40 kDa fragment (or doublet) (3). Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer (4).
Molecular Weight 69/40 kDA
Gene ID 2641
UniProt Q13421
Research Area Cell Signaling, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-10000
Comment

Background: Mesothelin is a tumor differentiation antigen that is present on the surface of normal mesothelial cells lining the pleura, peritoneum and pericardium. It is over expressed in many human tumors, including malignant mesothelioma and ovarian, pancreatic and lung adenocarcinomas (1). The normal biologic function of mesothelin is unknown but recent studies have shown that it forms a strong and specific complex with MUC16, a binding which has been suggested to be the basis of ovarian cancer metastasis (2). Initially, mesothelin is produced as a polypeptide with a hydrophobic tail which is removed and replaced by phosphatidylinositol. After glycosylation at the one or more of its four putative N-linked glycosylation sites, it is cleaved by a protease to yield the 40 kDa fragment (or doublet) (3). Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Mesothelin Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Chang, Pastan: "Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 1, pp. 136-40, 1996 (PubMed).

Hassan, Ho: "Mesothelin targeted cancer immunotherapy." in: European journal of cancer (Oxford, England : 1990), Vol. 44, Issue 1, pp. 46-53, 2007 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).

Sathyanarayana, Hahn, Patankar et al.: "Mesothelin, Stereocilin, and Otoancorin are predicted to have superhelical structures with ARM-type repeats." in: BMC structural biology, Vol. 9, pp. 1, 2009 (PubMed).

Hosts (55), (30), (7), (3)
Reactivities (80), (12), (6), (2), (1), (1)
Applications (51), (37), (24), (13), (10), (10), (7), (6), (6), (4), (4), (4), (4), (3), (2)
Conjugates (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (9), (8), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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