Dual Specificity Phosphatase 6 (DUSP6) antibody

Details for Product No. ABIN650057
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms fd19d04, zgc:55957, zgc:77211, wu:fd19d04, Mkp3, wu:fd99d09, mkp3, pyst1, DUSP6, dusp6, HH19, MKP3, PYST1, 1300019I03Rik, MKP-3
Reactivity
Human, Rat (Rattus)
(80), (31), (30), (13), (13)
Host
Rabbit
(63), (13), (3), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(64), (40), (25), (16), (11), (10), (9), (2), (2), (1)
Pubmed 2 references available
Quantity 100 μL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN650057
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic peptide corresponding to residues near the C-terminus of human MKP-3 was used as an immunogen.
Alternative Name MKP-3
Background MKP-3 is expressed constitutively in human skin fibroblasts and dephosphorylates and inactivates MAP kinase in vitro and in vivo. MKP-3 displays very low activity towards the stress-activated protein kinases (SAPKs) or RK/p38 in vitro, indicating that these kinases are not physiological substrates for MKP-3. MKP-3 is thought to play a role in the dephosphorylation and inactivation of MAP kinases and are therefore likely to be important in the regulation of diverse cellular processes such as proliferation, differentiation, and apoptosis. For this reason it has been suggested that MAP kinase phosphatases may be tumor suppressors (1). MKP-3 is expressed in lung, heart, brain, and kidney, but not significantly in skeletal muscle or testis. In situ hybridization studies of MKP-3 in brain reveal enrichment within the CA1, CA3, and CA4 layers of the hippocampus. Observations indicate that MKP-3 is a novel dual-specificity phosphatase that displays a distinct tissue distribution, subcellular localization, and regulated expression, suggesting a unique function in controlling MAP kinase family members (2).
Synonyms: DUSP6, MKP3, PYST1, Dual specificity protein phosphatase 6, Dual specificity protein phosphatase PYST1
Molecular Weight 42/44 kDA
Gene ID 1848
UniProt Q16828
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure
Application Notes IHC: = 1:50-100, WB: = 1:500
Comment

Background: MKP-3 is expressed constitutively in human skin fibroblasts and dephosphorylates and inactivates MAP kinase in vitro and in vivo. MKP-3 displays very low activity towards the stress-activated protein kinases (SAPKs) or RK/p38 in vitro, indicating that these kinases are not physiological substrates for MKP-3. MKP-3 is thought to play a role in the dephosphorylation and inactivation of MAP kinases and are therefore likely to be important in the regulation of diverse cellular processes such as proliferation, differentiation, and apoptosis. For this reason it has been suggested that MAP kinase phosphatases may be tumor suppressors (1). MKP-3 is expressed in lung, heart, brain, and kidney, but not significantly in skeletal muscle or testis. In situ hybridization studies of MKP-3 in brain reveal enrichment within the CA1, CA3, and CA4 layers of the hippocampus. Observations indicate that MKP-3 is a novel dual-specificity phosphatase that displays a distinct tissue distribution, subcellular localization, and regulated expression, suggesting a unique function in controlling MAP kinase family members (2).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment MKP-3 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Groom, Sneddon, Alessi et al.: "Differential regulation of the MAP, SAP and RK/p38 kinases by Pyst1, a novel cytosolic dual-specificity phosphatase." in: The EMBO journal, Vol. 15, Issue 14, pp. 3621-32, 1996 (PubMed).

Smith, Price, Cullen et al.: "Chromosomal localization of three human dual specificity phosphatase genes (DUSP4, DUSP6, and DUSP7)." in: Genomics, Vol. 42, Issue 3, pp. 524-7, 1997 (PubMed).

Validation Images
Did you look for something else?
back to top