MRP14/S100-A9 antibody

Antigen: MRP14/S100-A9

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN650083

Additional Information

Gene ID: 928

UniProt: P06702

Description: Other names: S100A9, CAGB, CFAG, MRP14, Protein S100-A9, Leukocyte L1 complex heavy chain,Calprotectin L1H subunit

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human MRP14/S100-A9 was used as an immunogen.

Molecular Weight: 14 kDA

Comments:

Background: S100-A9 (MRP14) is a member of the S100 family proteins containing two EF-hand calcium-binding motifs (1). S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation (2). S100-A9 forms a heterodimer, Calprotectin, with S100-A8 in a calcium-dependent manner. Calprotectin is a major calcuim- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes (3). S100-A9 may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis (2).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~5000 ICC: ~~ 1:100~250 IHC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: MRP14/S100-A9 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Hunter, Chazin: "High level expression and dimer characterization of the S100 EF-hand proteins, migration inhibitory factor-related proteins 8 and 14." in: The Journal of biological chemistry, Vol. 273, Issue 20, pp. 12427-35, 1998 (PubMed).

Sampson, Fagerhol, Sunderkötter et al.: "Hyperzincaemia and hypercalprotectinaemia: a new disorder of zinc metabolism." in: Lancet, Vol. 360, Issue 9347, pp. 1742-5, 2002 (PubMed).

Pruitt, Tatusova, Maglott: "NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins." in: Nucleic acids research, Vol. 35, Issue Database issue, pp. D61-5, 2007 (PubMed).