Myosin Light Chain, phosphorylatable, Fast Skeletal Muscle (MYLPF) antibody

Details for Product No. ABIN650101
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Antigen
Synonyms SkMLC, MYLPF, 2410014J02Rik, MLC-2, Mlc2, HUMMLC2B, MRLC2, MYL11, MLC2, Myl2, Myolc1, DTNB, G2, LC2f, CMH10
Reactivity
Human, Rat (Rattus)
(31), (14), (13), (1)
Host
Rabbit
(25), (8)
Clonality
Monoclonal
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(24), (11), (6), (5), (4), (2), (1), (1)
Pubmed 4 references available
Quantity 100 μL
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Catalog No. ABIN650101
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Specificity A synthetic peptide corresponding to residues in human Myosin Light Chain 2 was used as an immunogen.
Alternative Name Myosin Light Chain 2
Background Myosin is a hexamer of 2 identical heavy chains and 2 pairs of light chains (1). The two pairs of light chains of muscle myosins are called essential light chains (ELC) and regulatory light chains (RLC). The light chains stabilize the long alpha helical neck of the myosin head (2). Myosin light chain-2 (MYL2), also known as the regulatory light chain of myosin, is an important protein involved in the regulation of myosin ATPase activity in smooth muscle (3). Calcium triggers the phosphorylation of MYL2 that in turn triggers contraction. The phosphorylation sites of MYL2 are mainly Serine 19 (monophosphorylation) and, in some conditions, both Threonine 18 and Serine 19 (diphosphorylation) (4). An increase in ventricular MYL2 is observed during myocardial hypertrophy in cardiac patients with valve stenosis (3).
Synonyms: MYL2, Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
Molecular Weight 18 kDA
Gene ID 2266
UniProt P10916
Research Area Signaling, Protein Modifications, Cell Structure
Application Notes IHC: = 1:100-250, WB: = 1:10000-50000
Comment

Background: Myosin is a hexamer of 2 identical heavy chains and 2 pairs of light chains (1). The two pairs of light chains of muscle myosins are called essential light chains (ELC) and regulatory light chains (RLC). The light chains stabilize the long alpha helical neck of the myosin head (2). Myosin light chain-2 (MYL2), also known as the regulatory light chain of myosin, is an important protein involved in the regulation of myosin ATPase activity in smooth muscle (3). Calcium triggers the phosphorylation of MYL2 that in turn triggers contraction. The phosphorylation sites of MYL2 are mainly Serine 19 (monophosphorylation) and, in some conditions, both Threonine 18 and Serine 19 (diphosphorylation) (4). An increase in ventricular MYL2 is observed during myocardial hypertrophy in cardiac patients with valve stenosis (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Myosin Light Chain 2 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Macera, Szabo, Wadgaonkar et al.: "Localization of the gene coding for ventricular myosin regulatory light chain (MYL2) to human chromosome 12q23-q24.3." in: Genomics, Vol. 13, Issue 3, pp. 829-31, 1992 (PubMed).

Ikebe: "Phosphorylation of a second site for myosin light chain kinase on platelet myosin." in: Biochemistry, Vol. 28, Issue 22, pp. 8750-5, 1990 (PubMed).

Sweeney: "Function of the N terminus of the myosin essential light chain of vertebrate striated muscle." in: Biophysical journal, Vol. 68, Issue 4 Suppl, pp. 112S-118S; discussion 118S-119S, 1995 (PubMed).

Poetter, Jiang, Hassanzadeh et al.: "Mutations in either the essential or regulatory light chains of myosin are associated with a rare myopathy in human heart and skeletal muscle." in: Nature genetics, Vol. 13, Issue 1, pp. 63-9, 1996 (PubMed).

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