NCK Adaptor Protein 1 (NCK1) antibody

Details for Product No. ABIN650109
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Antigen
Synonyms NCK1, NCK, NCKalpha, nck-1, 6330586M15Rik, D230010O13Rik, Nck, Nck-1
Reactivity
Human, Rat (Rattus)
(53), (9), (9), (4)
Host
Rabbit
(37), (16), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(53), (28), (20), (8), (8), (5), (4), (3), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 µL
Options
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Catalog No. ABIN650109
450.00 $
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Specificity A synthetic peptide corresponding to residues close to the SH2 domain of human Nck was used as immunogen.
Alternative Name Nck
Background Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3 Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) leads to phosphorylation of Nck on tyrosine, serine and threonine residues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast (1-2). Additionally, Nck has been shown to associate with BLNK, PLCG1 and VAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linking tyrosine and serine/threonine kinase pathways within the cell (2).
Synonyms: NCK1, NCK, Cytoplasmic protein NCK1, SH2/SH3 adaptor protein NCK-alpha
Molecular Weight 47 kDA
Gene ID 4690
UniProt P16333
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes IHC: = 1:100-250, WB: = 1:500-2000
Comment

Background: Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3 Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) leads to phosphorylation of Nck on tyrosine, serine and threonine residues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast (1-2). Additionally, Nck has been shown to associate with BLNK, PLCG1 and VAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linking tyrosine and serine/threonine kinase pathways within the cell (2).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Nck Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Li, Hu, Skolnik et al.: "The SH2 and SH3 domain-containing Nck protein is oncogenic and a common target for phosphorylation by different surface receptors." in: Molecular and cellular biology, Vol. 12, Issue 12, pp. 5824-33, 1992 (PubMed).

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