NCK Adaptor Protein 1 (NCK1) antibody

Antigen: NCK Adaptor Protein 1 (NCK1)

Synonyms: NCK, MGC12668, NCKalpha, NCK1, MGC139414, DKFZp469N0721

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650109

Additional Information

Alternative name: Nck

Gene ID: 4690

UniProt: P16333

Description: Other names: NCK1, NCK, Cytoplasmic protein NCK1, SH2/SH3 adaptor protein NCK-alpha

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues close to the SH2 domain of human Nck was used as immunogen.

Molecular Weight: 47 kDA

Comments:

Background: Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3 Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) leads to phosphorylation of Nck on tyrosine, serine and threonine residues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast (1-2). Additionally, Nck has been shown to associate with BLNK, PLCG1 and VAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linking tyrosine and serine/threonine kinase pathways within the cell (2).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500~2000 IP: ~~ 1:50 FC: ~~ 1:60 ICC: ~~ 1:250~500 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Nck Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Translation Factors, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Li, Hu, Skolnik et al.: "The SH2 and SH3 domain-containing Nck protein is oncogenic and a common target for phosphorylation by different surface receptors." in: Molecular and cellular biology, Vol. 12, Issue 12, pp. 5824-33, 1992 (PubMed).