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Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term) antibody

Details for Product No. ABIN650112
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Antigen
Synonyms NEDD-8, Rub1, CG10679, Dmel\\CG10679, NEDD8, dNEDD8, nedd8, ubiquitin, si:zc14a17.5, GB17785, DDBDRAFT_0218116, DDBDRAFT_0238041, DDB_0218116, DDB_0238041
Epitope
N-Term
(20), (3), (2), (1), (1), (1), (1)
Reactivity
Human
(67), (36), (35), (12), (12), (1)
Host
Rabbit
(55), (12), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(59), (38), (30), (14), (11), (10), (8), (8), (3), (3), (2), (1)
Pubmed 3 references available
Quantity 100 µL
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Availability Will be delivered in 2 to 3 Business Days
Catalog No. ABIN650112
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic peptide corresponding to residues in the N-term of human NEDD8 was used as immunogen. This antibody detects NEDD8 bound to cullins proteins (neddylated cullins) as well as free NEDD8.
Alternative Name NEDD8
Background NEDD8 (Neural precursor cell-Expressed Developmentally Down-regulated genes) is an ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis (1). NEDD8 has been shown to conjugate to nuclear proteins in a manner analogous to ubiquination and sentrinization. The conjugation process is though to be catalyzed by thee enzymes, E1 (NEDD8-activation), E2 (NEDD8-conjugation) and E3 (NEDD8-ligation). NEDD8 is known to conjugate to Cul-4A and other cullins proteins via the carboxy-terminal Gly residue for ubiquitinylation (3) while NUB1 will negatively regulate its expression (4).
Synonyms: NEDD8, NEDD8, Ubiquitin-like protein Nedd8,Neddylin,Neural precursor cell expressed developmentally down-regulated protein 8
Molecular Weight 10/90 kDA
Gene ID 4738
UniProt Q15843
Research Area Signaling, Protein Modifications, Cell Structure
Application Notes IHC: = 1:100-250, WB: = 1:500-1000
Comment

Background: NEDD8 (Neural precursor cell-Expressed Developmentally Down-regulated genes) is an ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis (1). NEDD8 has been shown to conjugate to nuclear proteins in a manner analogous to ubiquination and sentrinization. The conjugation process is though to be catalyzed by thee enzymes, E1 (NEDD8-activation), E2 (NEDD8-conjugation) and E3 (NEDD8-ligation). NEDD8 is known to conjugate to Cul-4A and other cullins proteins via the carboxy-terminal Gly residue for ubiquitinylation (3) while NUB1 will negatively regulate its expression (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment NEDD8 Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Kumar, Yoshida, Noda: "Cloning of a cDNA which encodes a novel ubiquitin-like protein." in: Biochemical and biophysical research communications, Vol. 195, Issue 1, pp. 393-9, 1993 (PubMed).

Kipreos, Lander, Wing et al.: "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family." in: Cell, Vol. 85, Issue 6, pp. 829-39, 1996 (PubMed).

Gong, Yeh: "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway." in: The Journal of biological chemistry, Vol. 274, Issue 17, pp. 12036-42, 1999 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN650112
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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