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Details for Product No. ABIN650112

Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term) antibody

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Antigen
Synonyms NEDD-8, Rub1, CG10679, Dmel\\CG10679, NEDD8, dNEDD8, nedd8, ubiquitin, si:zc14a17.5, GB17785, DDBDRAFT_0218116, DDBDRAFT_0238041, DDB_0218116, DDB_0238041
Epitope
»Alternatives N-Term
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Pubmed 3 references available
Catalog no. ABIN650112
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic peptide corresponding to residues in the N-term of human NEDD8 was used as immunogen. This antibody detects NEDD8 bound to cullins proteins (neddylated cullins) as well as free NEDD8.
Alternative Name NEDD8
Background NEDD8 (Neural precursor cell-Expressed Developmentally Down-regulated genes) is an ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis (1). NEDD8 has been shown to conjugate to nuclear proteins in a manner analogous to ubiquination and sentrinization. The conjugation process is though to be catalyzed by thee enzymes, E1 (NEDD8-activation), E2 (NEDD8-conjugation) and E3 (NEDD8-ligation). NEDD8 is known to conjugate to Cul-4A and other cullins proteins via the carboxy-terminal Gly residue for ubiquitinylation (3) while NUB1 will negatively regulate its expression (4).
Synonyms: NEDD8, NEDD8, Ubiquitin-like protein Nedd8,Neddylin,Neural precursor cell expressed developmentally down-regulated protein 8
Molecular Weight 10/90 kDA
Gene ID 4738
UniProt Q15843
Research Area Signaling, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:500~1000 IP: ~~ 1:50 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250
Comment

Background: NEDD8 (Neural precursor cell-Expressed Developmentally Down-regulated genes) is an ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis (1). NEDD8 has been shown to conjugate to nuclear proteins in a manner analogous to ubiquination and sentrinization. The conjugation process is though to be catalyzed by thee enzymes, E1 (NEDD8-activation), E2 (NEDD8-conjugation) and E3 (NEDD8-ligation). NEDD8 is known to conjugate to Cul-4A and other cullins proteins via the carboxy-terminal Gly residue for ubiquitinylation (3) while NUB1 will negatively regulate its expression (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment NEDD8 Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Kumar, Yoshida, Noda: "Cloning of a cDNA which encodes a novel ubiquitin-like protein." in: Biochemical and biophysical research communications, Vol. 195, Issue 1, pp. 393-9, 1993 (PubMed).

Kipreos, Lander, Wing et al.: "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family." in: Cell, Vol. 85, Issue 6, pp. 829-39, 1996 (PubMed).

Gong, Yeh: "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway." in: The Journal of biological chemistry, Vol. 274, Issue 17, pp. 12036-42, 1999 (PubMed).

Alternatives for antigen "Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8)", type "Antibodies"
Hosts (45), (11), (4)
Reactivities (55), (31), (30), (12), (12), (1)
Applications (49), (29), (19), (17), (10), (9), (7), (6), (5), (4), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (13), (3), (2), (1), (1), (1)
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