Neurofilament L (C-Term) antibody

Antigen: Neurofilament L

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650115

Additional Information

Gene ID: 4747

UniProt: P07196

Description: Other names: NEFM, NEF3, NFM, Neurofilament medium polypeptide, Neurofilament triplet M protein,160 kDa neurofilament protein,Neurofilament 3

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the C-term of human NF-L was used as immunogen.

Molecular Weight: 68 kDA

Comments:

Background: Neuofilaments (NF) are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton (1). NFs are composed of three major proteins, NF-H, NF-L and NF-M and the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein (200 kDa), NF-M the medium (160 kDa) and NF-L the light protein (68kDa) (2). NF-L self polymerizes and provides a scaffold for NFs to form via cross-bridging with NF-H or NF-M (3). NF-L aggregation has been linked to the pathogenesis of neurodegenerative disease, such as degradation of motor neurons (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000 IP: ~~ 1:50 FC: ~~ 1:70

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Neurofilament L Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Neurology, Cell Structure

Restrictions: For Research Use only

Publications

Product: Julien, Meyer, Flavell et al.: "Cloning and developmental expression of the murine neurofilament gene family." in: Brain research, Vol. 387, Issue 3, pp. 243-50, 1987 (PubMed).