Add to Basket Bulk discount
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

Neurofilament Triplet M Protein (NEFM) antibody

Antigen

Neurofilament Triplet M Protein (NEFM)
Synonyms NFM, NEF3, NF-M, Nfm, Nef3, LOC787355
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN650116
Quantity 100ul
Price 450.00 $   Plus shipping costs $45.00
Bulk discount
Shipping to
Availability Will be delivered in 2 to 3 Business Days

Additional Information
Application Details Publications Alternatives back to top

Alternative name Neurofilament M
Gene ID 6291
UniProt P07197
Description Other names: NEFM, NEF3, NFM, Neurofilament medium polypeptide, Neurofilament triplet M protein,160 kDa neurofilament protein,Neurofilament 3
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues in human Neurofilament M was used as an immunogen.
Molecular Weight 160 kDA
Comments

Background: Neuofilaments (NF) are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton (1). NFs are composed of three major proteins, NF-H, NF-M and NF-L and the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein (200 kDa), NF-M the medium (160 kDa) and NF-L the light protein (68kDa) (2).

Application Details
Publications Alternatives back to top

Application Notes The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:10 ICC: ~~ 1:100~250 IHC: ~~ 1:250~500
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Neurofilament M Antibody can be stored at -20°C for up to 12 months from time of receipt.
Research Area Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Nixon, Shea: "Dynamics of neuronal intermediate filaments: a developmental perspective." in: Cell motility and the cytoskeleton, Vol. 22, Issue 2, pp. 81-91, 1992 (PubMed).

Julien, Meyer, Flavell et al.: "Cloning and developmental expression of the murine neurofilament gene family." in: Brain research, Vol. 387, Issue 3, pp. 243-50, 1987 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Neurofilament Triplet M Protein (NEFM)", type "Antibodies"
Hosts Rabbit (35), Mouse (34), Chicken (11), Goat (2)
Reactivities Rat (Rattus) (50), Human (43), Mouse (Murine) (31), Cow (Bovine) (25), Pig (Porcine) (22), Chicken (6), Cat (Feline) (5), Hamster (2), Monkey (2), Rabbit (2), Xenopus laevis (2), Bird (Avian) (1), Dog (Canine) (1), Guinea Pig (1), Primate (1)
Applications Western Blotting (WB) (62), Immunofluorescence (IF) (36), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (23), ELISA (19), Immunohistochemistry (Fixed) (IHC (fx)) (15), Immunocytochemistry (ICC) (13), Immunohistochemistry (IHC) (13), Flow Cytometry (FACS) (6), Immunoprecipitation (IP) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunoelectron Microscopy (IEM) (1), Radioimmunoassay (RIA) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes C-Term (19), Internal Region (4), pSer614, pSer619 (1)