Neurofilament Triplet M Protein (NEFM) (pSer614, pSer619) antibody

Antigen: Neurofilament Triplet M Protein (NEFM)

Synonyms: NFM, NEF3, NF-M, Nfm, Nef3, LOC787355

Binding Site: pSer614, pSer619

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650117

Additional Information

Alternative name: Neurofilament M

Gene ID: 4741

UniProt: P07197

Description: Other names: NEFM, NEF3, NFM, Neurofilament medium polypeptide, Neurofilament triplet M protein,160 kDa neurofilament protein,Neurofilament 3

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic phospho-peptide corresponding to residues surrounding Serine 614/619 of human NF-M was used as an immunogen.

Molecular Weight: 160 kDA

Comments:

Background: Neuofilaments (NF) are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton (1). NFs are composed of three major proteins, NF-H, NF-L and NF-M and the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein (200 kDa), NF-M the medium (160 kDa) and NF-L the light protein (68kDa) (2). Several phsopholyation sites have been discovered on NF-M, including serine 614 and 619. However, their specific implication on NF-M function has not been identified.

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500 IP: ~~ 1:30 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Neurofilament M Antibody Phospho (pS614/619) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Julien, Meyer, Flavell et al.: "Cloning and developmental expression of the murine neurofilament gene family." in: Brain research, Vol. 387, Issue 3, pp. 243-50, 1987 (PubMed).