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Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) antibody

Details for Product No. ABIN650121
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Antigen
Synonyms
ebp-1, kbf1, nf-kappa-b, nf-kappaB, nfkb-p105, nfkb-p50, NF-KB1, NF-kappaB, NF-kappaB1, p105, p50, p50/p105, EBP-1, KBF1, NF-kB1, NF-kappa-B, NFKB-p105, NFKB-p50, NFkappaB, BICDR-1, bicdr1, ccdc64, NF ... show more
ebp-1, kbf1, nf-kappa-b, nf-kappaB, nfkb-p105, nfkb-p50, NF-KB1, NF-kappaB, NF-kappaB1, p105, p50, p50/p105, EBP-1, KBF1, NF-kB1, NF-kappa-B, NFKB-p105, NFKB-p50, NFkappaB, BICDR-1, bicdr1, ccdc64, NF-kB show less
Reactivity
Human, Rat (Rattus)
(431), (172), (139), (63), (52), (27), (27), (1), (1), (1)
Host
Rabbit
(417), (38), (2), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(13), (11), (11), (8), (8), (8), (8), (8), (8), (8), (8), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(344), (178), (151), (99), (80), (70), (36), (30), (12), (7), (7), (7), (4), (2), (2), (2), (1), (1)
Pubmed 2 references available
Quantity 100 µL
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Catalog No. ABIN650121
450.00 $
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Specificity A synthetic peptide corresponding to residues in the nuclear factor NF-κ,B p50 subunit was used as immunogen. This antibody will detect both forms, p50 and p105.
Alternative Name NF-kappa B (p105/p50)
Background Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes (1). The family members include p50, p52, p65 (RelA), c-Rel, and RelB (2). Association with inhibitory proteins of the Iκ,B family retains NF-κ,B in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-B to DNA (3). The p105 precursor protein acts as an NFκ,B inhibitor, retaining Rel subunits in the cytoplasm of unstimulated cells. Upon stimulation by TNFα, and IL -1α,, degradation of p105 occurs, the Rel subunit is released and translocats to the nucleus (4). Additionally, p105 will be proteolytically processed by the 26S proteasome to produce a smaller, transcriptionally active form (p50) (5).
Synonyms: NFKB1, Nuclear factor NF-kappa-B p50 subunit, DNA-binding factor KBF1,EBP-1
Molecular Weight 105/50 kDA
Gene ID 4790
UniProt P19838
Research Area Ubiquitin-related antibodies, Apoptosis/Necrosis, Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure
Application Notes IHC: = 1:250-500, WB: = 1:500-1000
Comment

Background: Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes (1). The family members include p50, p52, p65 (RelA), c-Rel, and RelB (2). Association with inhibitory proteins of the Iκ,B family retains NF-κ,B in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-B to DNA (3). The p105 precursor protein acts as an NFκ,B inhibitor, retaining Rel subunits in the cytoplasm of unstimulated cells. Upon stimulation by TNFα, and IL -1α,, degradation of p105 occurs, the Rel subunit is released and translocats to the nucleus (4). Additionally, p105 will be proteolytically processed by the 26S proteasome to produce a smaller, transcriptionally active form (p50) (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment NF-κ,B Antibody (p105/p50) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Verma, Stevenson, Schwarz et al.: "Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation." in: Genes & development, Vol. 9, Issue 22, pp. 2723-35, 1995 (PubMed).

Palombella, Rando, Goldberg et al.: "The ubiquitin-proteasome pathway is required for processing the NF-kappa B1 precursor protein and the activation of NF-kappa B." in: Cell, Vol. 78, Issue 5, pp. 773-85, 1994 (PubMed).

Validation Images
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