Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) antibody

Antigen: Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1)

Synonyms: p50, KBF1, p105, EBP-1, MGC54151, NFKB-p50, NF-kappaB, NFKB-p105, NF-kappa-B, DKFZp686C01211, NF-kB, NF-KB1, NFKB1, kbf1, ebp-1, MGC86262, nfkb-p50, nf-kappaB, nfkb-p105, nf-kappa-b, p52, LYT10, LYT-10

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN650121

Additional Information

Alternative name: NF-kappa B (p105/p50)

Gene ID: 4790

UniProt: P19838

Description: Other names: NFKB1, Nuclear factor NF-kappa-B p50 subunit, DNA-binding factor KBF1,EBP-1

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in the nuclear factor NF-κ,B p50 subunit was used as immunogen. This antibody will detect both forms, p50 and p105.

Molecular Weight: 105/50 kDA

Comments:

Background: Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes (1). The family members include p50, p52, p65 (RelA), c-Rel, and RelB (2). Association with inhibitory proteins of the Iκ,B family retains NF-κ,B in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-B to DNA (3). The p105 precursor protein acts as an NFκ,B inhibitor, retaining Rel subunits in the cytoplasm of unstimulated cells. Upon stimulation by TNFα, and IL -1α,, degradation of p105 occurs, the Rel subunit is released and translocats to the nucleus (4). Additionally, p105 will be proteolytically processed by the 26S proteasome to produce a smaller, transcriptionally active form (p50) (5).

Synonyms: p50, KBF1, p105, EBP-1, MGC54151, NFKB-p50, NF-kappaB, NFKB-p105, NF-kappa-B, DKFZp686C01211, NF-kB, NF-KB1, NFKB1, kbf1, ebp-1, MGC86262, nfkb-p50, nf-kappaB, nfkb-p105, nf-kappa-b, p52, LYT10, LYT-10

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500~1000 IP: ~~ 1:50 FC: ~~ 1:40 ICC: ~~ 1:250~500 IHC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: NF-κ,B Antibody (p105/p50) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Apoptosis/Necrosis, Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Publications

Product: Verma, Stevenson, Schwarz et al.: "Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation." in: Genes & development, Vol. 9, Issue 22, pp. 2723-35, 1995 (PubMed).

Palombella, Rando, Goldberg et al.: "The ubiquitin-proteasome pathway is required for processing the NF-kappa B1 precursor protein and the activation of NF-kappa B." in: Cell, Vol. 78, Issue 5, pp. 773-85, 1994 (PubMed).