NF-kappa-B p65 antibody

Antigen: NF-kappa-B p65

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN650125

Additional Information

Gene ID: 5970

UniProt: Q04206

Description: Other names: RELA, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic phospho-peptide corresponding to residues surrounding serine 486 of human NF-kappa-B protein. This antibody detects both phosphorylated and non-phosphorylated version of human NF-kappa-B protein

Molecular Weight: 70 kDA

Comments:

Background: Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes (1). The family members include p50, p52, p65 (RelA), c-Rel, and RelB. (2). The p65 subunit, similar to two others in the B family, RelB and c-Rel, contains two transactivation domains in the C-terminal region of the protein (3). Association with inhibitory proteins of the IkappaB family retains NF-kappaB in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-B to DNA (4). A phosphorylation at serine 468 of NF-B by GSK3 beta and IKK beta has been linked negative regulation of NF-B without the inhibition of NF-B nuclear translocation after TNF-alpha or IL-1 beta stimulation (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 FC: ~~ 1:30 ICC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: NF-kappa-B p65 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Publications

Product: Verma, Stevenson, Schwarz et al.: "Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation." in: Genes & development, Vol. 9, Issue 22, pp. 2723-35, 1995 (PubMed).

Schmitz, dos Santos Silva, Baeuerle: "Transactivation domain 2 (TA2) of p65 NF-kappa B. Similarity to TA1 and phorbol ester-stimulated activity and phosphorylation in intact cells." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15576-84, 1995 (PubMed).

Baldwin: "The NF-kappa B and I kappa B proteins: new discoveries and insights." in: Annual review of immunology, Vol. 14, Issue 8, pp. 649-83, 1996 (PubMed).

Jacobs, Harrison: "Structure of an IkappaBalpha/NF-kappaB complex." in: Cell, Vol. 95, Issue 6, pp. 749-58, 1999 (PubMed).