Nuclear Factor-KB P65 (NFkBP65) antibody

Details for Product No. ABIN650125
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms c-Rel, C-Rel, Xrel2, c-rel, rel-A, v-rel, xrel, NFKB3, p65, NFkB, zgc:100833
Reactivity
Human
(854), (445), (354), (76), (75), (74), (38), (9), (8), (6), (2), (2), (1), (1)
Host
Rabbit
(799), (67), (6), (5), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(24), (19), (15), (11), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (1), (1), (1)
Application
Western Blotting (WB)
(655), (336), (332), (170), (129), (123), (100), (38), (21), (18), (16), (12), (7), (5), (2), (1)
Pubmed 4 references available
Quantity 100 μL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN650125
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic phospho-peptide corresponding to residues surrounding serine 486 of human NF-kappa-B protein. This antibody detects both phosphorylated and non-phosphorylated version of human NF-kappa-B protein
Alternative Name NF-kappa B p65
Background Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes (1). The family members include p50, p52, p65 (RelA), c-Rel, and RelB. (2). The p65 subunit, similar to two others in the B family, RelB and c-Rel, contains two transactivation domains in the C-terminal region of the protein (3). Association with inhibitory proteins of the IkappaB family retains NF-kappaB in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-B to DNA (4). A phosphorylation at serine 468 of NF-B by GSK3 beta and IKK beta has been linked negative regulation of NF-B without the inhibition of NF-B nuclear translocation after TNF-alpha or IL-1 beta stimulation (5).
Synonyms: RELA, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit
Molecular Weight 70 kDA
Gene ID 5970
UniProt Q04206
Research Area Ubiquitin-related antibodies, Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-10000
Comment

Background: Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes (1). The family members include p50, p52, p65 (RelA), c-Rel, and RelB. (2). The p65 subunit, similar to two others in the B family, RelB and c-Rel, contains two transactivation domains in the C-terminal region of the protein (3). Association with inhibitory proteins of the IkappaB family retains NF-kappaB in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-B to DNA (4). A phosphorylation at serine 468 of NF-B by GSK3 beta and IKK beta has been linked negative regulation of NF-B without the inhibition of NF-B nuclear translocation after TNF-alpha or IL-1 beta stimulation (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment NF-kappa-B p65 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Verma, Stevenson, Schwarz et al.: "Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation." in: Genes & development, Vol. 9, Issue 22, pp. 2723-35, 1995 (PubMed).

Schmitz, dos Santos Silva, Baeuerle: "Transactivation domain 2 (TA2) of p65 NF-kappa B. Similarity to TA1 and phorbol ester-stimulated activity and phosphorylation in intact cells." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15576-84, 1995 (PubMed).

Baldwin: "The NF-kappa B and I kappa B proteins: new discoveries and insights." in: Annual review of immunology, Vol. 14, Issue 8, pp. 649-83, 1996 (PubMed).

Jacobs, Harrison: "Structure of an IkappaBalpha/NF-kappaB complex." in: Cell, Vol. 95, Issue 6, pp. 749-58, 1999 (PubMed).

Validation Images
Did you look for something else?
back to top