Nibrin (NBN) (pSer343) antibody

Details for Product No. ABIN650131
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Antigen
Synonyms NBN, AT-V1, AT-V2, ATV, NBS, NBS1, P95, Nbs1, im:6911679, zgc:194152
Epitope
pSer343
(47), (20), (8), (8), (7), (6), (6), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(221), (81), (55), (24), (24), (24)
Host
Rabbit
(178), (47)
Clonality
Monoclonal
Conjugate
Un-conjugated
(7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application
Western Blotting (WB)
(183), (76), (46), (40), (35), (27), (24), (22), (12), (2), (2), (1)
Pubmed 2 references available
Quantity 100 μL
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Catalog No. ABIN650131
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Specificity A phospho-specific peptide corresponding to residues surrounding Serine 343 in human Nibrin/NBS1 was used as an immunogen. The antibody only detects Nibrin/NBS1 phosphorylated on Serine 343.
Alternative Name Nibrin/NBS1
Background Nibrin (Nijmegen breakage syndrome protein 1, NBS1) contains 2 modules found in cell cycle check point, a forkhead-associated domain and a BRCA1 C-teminal repeat (1). In mammalian cells, Nibrin forms a complex with Mre11 and Rad50 (MRN). Nuclear localization of the MRN complex is relevant for chromosomal stability, meiotic recombination, DNA repair and telomere maintenance in eukaryotic cells. Mutation in Nibrin and Mre11 causes respectively two related disorders, Nijmegen breakage syndrome (NBS) and Ataxia-Telangiectasia- like disorder (ATLD) (2-4). Nibrin is phosphorylated at Serine 343 by ATM. The in vivo modification of these residues is essential for the cellular response to DNA damage, including an S-phase checkpoint activation, formation of the NBS1/Mrel1/Rad50 nuclear foci and rescue of hypersensitivity to ionizing radiation (5).
Synonyms: NBN, NBS, NBS1, P95, Nibrin, Nijmegen breakage syndrome protein 1,Cell cycle regulatory protein p95
Molecular Weight 95 kDA
Gene ID 4683
UniProt O60934
Research Area Phospho-specific antibodies, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:5000
Comment

Background: Nibrin (Nijmegen breakage syndrome protein 1, NBS1) contains 2 modules found in cell cycle check point, a forkhead-associated domain and a BRCA1 C-teminal repeat (1). In mammalian cells, Nibrin forms a complex with Mre11 and Rad50 (MRN). Nuclear localization of the MRN complex is relevant for chromosomal stability, meiotic recombination, DNA repair and telomere maintenance in eukaryotic cells. Mutation in Nibrin and Mre11 causes respectively two related disorders, Nijmegen breakage syndrome (NBS) and Ataxia-Telangiectasia- like disorder (ATLD) (2-4). Nibrin is phosphorylated at Serine 343 by ATM. The in vivo modification of these residues is essential for the cellular response to DNA damage, including an S-phase checkpoint activation, formation of the NBS1/Mrel1/Rad50 nuclear foci and rescue of hypersensitivity to ionizing radiation (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Nibrin/NBS1 Antibody Phospho (pS343) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Trujillo, Yuan, Lee et al.: "Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95." in: The Journal of biological chemistry, Vol. 273, Issue 34, pp. 21447-50, 1998 (PubMed).

Zhao, Weng, Yuan et al.: "Functional link between ataxia-telangiectasia and Nijmegen breakage syndrome gene products." in: Nature, Vol. 405, Issue 6785, pp. 473-7, 2000 (PubMed).

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