Nibrin (NBN) (pSer343) antibody

Antigen: Nibrin (NBN)

Synonyms: ATV, NBS, P95, NBS1, AT-V1, AT-V2, FLJ10155, MGC87362, Nbs1, NBN, DKFZp469A0911

Binding Site: pSer343

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650131

Additional Information

Alternative name: Nibrin/NBS1

Gene ID: 4683

UniProt: O60934

Description: Other names: NBN, NBS, NBS1, P95, Nibrin, Nijmegen breakage syndrome protein 1,Cell cycle regulatory protein p95

Characteristics: Rabbit Monoclonal Antibody

Specificity: A phospho-specific peptide corresponding to residues surrounding Serine 343 in human Nibrin/NBS1 was used as an immunogen. The antibody only detects Nibrin/NBS1 phosphorylated on Serine 343.

Molecular Weight: 95 kDA

Comments:

Background: Nibrin (Nijmegen breakage syndrome protein 1, NBS1) contains 2 modules found in cell cycle check point, a forkhead-associated domain and a BRCA1 C-teminal repeat (1). In mammalian cells, Nibrin forms a complex with Mre11 and Rad50 (MRN). Nuclear localization of the MRN complex is relevant for chromosomal stability, meiotic recombination, DNA repair and telomere maintenance in eukaryotic cells. Mutation in Nibrin and Mre11 causes respectively two related disorders, Nijmegen breakage syndrome (NBS) and Ataxia-Telangiectasia- like disorder (ATLD) (2-4). Nibrin is phosphorylated at Serine 343 by ATM. The in vivo modification of these residues is essential for the cellular response to DNA damage, including an S-phase checkpoint activation, formation of the NBS1/Mrel1/Rad50 nuclear foci and rescue of hypersensitivity to ionizing radiation (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:5000 IP: ~~ 1:50 ICC: ~~ 1:50~100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Nibrin/NBS1 Antibody Phospho (pS343) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Trujillo, Yuan, Lee et al.: "Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95." in: The Journal of biological chemistry, Vol. 273, Issue 34, pp. 21447-50, 1998 (PubMed).

Zhao, Weng, Yuan et al.: "Functional link between ataxia-telangiectasia and Nijmegen breakage syndrome gene products." in: Nature, Vol. 405, Issue 6785, pp. 473-7, 2000 (PubMed).