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Nibrin (NBN) (pSer343) antibody
|Synonyms||ATV, NBS, P95, NBS1, AT-V1, AT-V2, FLJ10155, MGC87362, Nbs1, NBN, DKFZp469A0911|
Alternatives Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
|2 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: NBN, NBS, NBS1, P95, Nibrin, Nijmegen breakage syndrome protein 1,Cell cycle regulatory protein p95|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A phospho-specific peptide corresponding to residues surrounding Serine 343 in human Nibrin/NBS1 was used as an immunogen. The antibody only detects Nibrin/NBS1 phosphorylated on Serine 343.|
|Molecular Weight||95 kDA|
Background: Nibrin (Nijmegen breakage syndrome protein 1, NBS1) contains 2 modules found in cell cycle check point, a forkhead-associated domain and a BRCA1 C-teminal repeat (1). In mammalian cells, Nibrin forms a complex with Mre11 and Rad50 (MRN). Nuclear localization of the MRN complex is relevant for chromosomal stability, meiotic recombination, DNA repair and telomere maintenance in eukaryotic cells. Mutation in Nibrin and Mre11 causes respectively two related disorders, Nijmegen breakage syndrome (NBS) and Ataxia-Telangiectasia- like disorder (ATLD) (2-4). Nibrin is phosphorylated at Serine 343 by ATM. The in vivo modification of these residues is essential for the cellular response to DNA damage, including an S-phase checkpoint activation, formation of the NBS1/Mrel1/Rad50 nuclear foci and rescue of hypersensitivity to ionizing radiation (5).
|Application Notes||The suggested dilution is: WB: ~~ 1:5000 IP: ~~ 1:50 ICC: ~~ 1:50~100|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Nibrin/NBS1 Antibody Phospho (pS343) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Phospho-specific antibodies, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Trujillo, Yuan, Lee et al.: "Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95." in: The Journal of biological chemistry, Vol. 273, Issue 34, pp. 21447-50, 1998 (PubMed).
Zhao, Weng, Yuan et al.: "Functional link between ataxia-telangiectasia and Nijmegen breakage syndrome gene products." in: Nature, Vol. 405, Issue 6785, pp. 473-7, 2000 (PubMed).