Nicastrin (NCSTN) antibody

Antigen: Nicastrin (NCSTN)

Synonyms: APH2, KIAA0253, RP11-517F10.1, Nct, Nic, S5A, agro, dNct, DmelCG7012, CG7012, Aph2, AA727311, D1Dau13e, Kiaa0253, mKIAA0253, 9430068N19Rik, NCSTN, MGC126920, DKFZp469O2418

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650135

Additional Information

Alternative name: Nicastrin

Gene ID: 23385

UniProt: Q92542

Description: Other names: NCSTN, KIAA0253, Nicastrin

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues on the C-terminus of human Nicastrin was used as an immunogen.

Molecular Weight: 120/140 (2 isoforms) kDA

Comments:

Background: Nicastrin, a transmembrane glycoprotein, is an essential component of the gamma-secretase complex that is required for the intramembrane proteolysis of an increasing number of membrane proteins, including the amyloid-beta precursor protein (APP) and Notch (1). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex (2). Nicastrin regulates gamma-secretase cleavage of the amyloid precursor protein by forming complexes with presenilins, in which most mutations causing familial early-onset Alzheimer's disease (EOAD) have been found (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000 IP: ~~ 1:40 FC: ~~ 1:20

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Nicastrin Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Ashby, Kehoe, Love: "Kallikrein-related peptidase 6 in Alzheimer's disease and vascular dementia." in: Journal of the American Chemical Society, Vol. 1363, Issue Nucleic acids research, pp. 1-10, 2010

Yu, Nishimura, Arawaka et al.: "Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing." in: Nature, Vol. 407, Issue 6800, pp. 48-54, 2000 (PubMed).

Dermaut, Theuns, Sleegers et al.: "The gene encoding nicastrin, a major gamma-secretase component, modifies risk for familial early-onset Alzheimer disease in a Dutch population-based sample." in: American journal of human genetics, Vol. 70, Issue 6, pp. 1568-74, 2002 (PubMed).

"The Universal Protein Resource (UniProt) in 2010." in: Nucleic acids research, Vol. 38, Issue Database issue, pp. D142-8, 2009 (PubMed).