Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (N-Term) antibody

Details for Product No. ABIN650144
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Antigen
Synonyms npm4, npm, B23, NPM, NO38, B23NP, Npm, CG7917, CRP1, Crp1, Dmel\\CG7917, dNLP, dNLP-S, p22
Epitope
N-Term
(30), (26), (13), (13), (12), (12), (7), (6), (6), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(216), (121), (119), (50), (49), (42), (15), (9), (7), (2), (2), (1), (1), (1), (1)
Host
Rabbit
(169), (64), (7), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(10), (7), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(191), (102), (70), (64), (62), (40), (37), (36), (18), (10), (3)
Pubmed 1 reference available
Quantity 100 µL
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Catalog No. ABIN650144
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Specificity A synthetic peptide corresponding to residues on the N-terminus of human NPM was used as an immunogen
Alternative Name NPM
Background Nucleophosmin (NPM), a nucleocytoplasmic shuttling protein with prominent nucleolar localization, regulates the ARF-p53 tumor-suppressor pathway. Translocations involving the NPM gene cause cytoplasmic dislocation of the NPM protein (1). NPM is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. Data suggest that transcription factor YY1 may play a role in NPM gene expression (2). A chromosomal translocation associated with myelodysplastic syndrome and acute myeloid leukemia (AML) was found to rearrange part of the nucleophosmin (NPM) gene. Myeloid leukemia factor 1 (MLF1) binds NPM and studies have indicated that MLF1 is normally located in the cytoplasm, whereas NPM-MLF1 is targeted to the nucleus, with highest levels in the nucleolus. The nuclear/nucleolar localization of NPM-MLF1 mirrors that of NPM, indicating that NPM trafficking signals direct MLF1 to an inappropriate cellular compartment in myeloid leukemia cells (3)
Synonyms: NPM1, NPM, Nucleophosmin, Nucleolar phosphoprotein B23,Numatrin,Nucleolar protein NO38
Molecular Weight 35/38 (2 isoforms) kDA
Gene ID 4869
UniProt P06748
Research Area Ubiquitin-related antibodies, Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Cancer, Chromatin, Cell Structure
Application Notes IHC: = 1:100-250, WB: = 1:200000
Comment

Background: Nucleophosmin (NPM), a nucleocytoplasmic shuttling protein with prominent nucleolar localization, regulates the ARF-p53 tumor-suppressor pathway. Translocations involving the NPM gene cause cytoplasmic dislocation of the NPM protein (1). NPM is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. Data suggest that transcription factor YY1 may play a role in NPM gene expression (2). A chromosomal translocation associated with myelodysplastic syndrome and acute myeloid leukemia (AML) was found to rearrange part of the nucleophosmin (NPM) gene. Myeloid leukemia factor 1 (MLF1) binds NPM and studies have indicated that MLF1 is normally located in the cytoplasm, whereas NPM-MLF1 is targeted to the nucleus, with highest levels in the nucleolus. The nuclear/nucleolar localization of NPM-MLF1 mirrors that of NPM, indicating that NPM trafficking signals direct MLF1 to an inappropriate cellular compartment in myeloid leukemia cells (3)

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment NPM Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Yoneda-Kato, Look, Kirstein et al.: "The t(3;5)(q25.1;q34) of myelodysplastic syndrome and acute myeloid leukemia produces a novel fusion gene, NPM-MLF1." in: Oncogene, Vol. 12, Issue 2, pp. 265-75, 1996 (PubMed).

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