Did you know that you can buy products from over 140 different suppliers from us?

POU Class 2 Associating Factor 1 (POU2AF1) antibody

Details for Product No. ABIN650151
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms BOB1, OBF-1, OBF1, OCAB, BOB-1, BOB.1, Bob-1, Bob1, OBF.1, OCA-B
Reactivity
Human
(72), (31), (30), (27), (26), (24), (12)
Host
Rabbit
(59), (12), (4)
Clonality
Monoclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(46), (31), (20), (19), (10), (9), (2)
Pubmed 2 references available
Quantity 100 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN650151
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic peptide corresponding to residues in human OCA-B was used as an immunogen.
Alternative Name OCA-B
Background OCA-B is a lymphoid-specific transcriptional coactivator which interacts with the transcription factors Oct-1 and Oct-2 (1). It has been shown to be critical for development of a normal immune response, where it mediates octamer-dependent transcriptional activity in B lymphocytes. It is also critically involved in the formation of germinal centers in secondary lymphoid organs. OCA-B levels have been observed to be massively upregulated in germinal center B cells, as compared with resting B cells. SIAH1 and SIAH2, known regulators of protein stability, have been identified as two related OCA-B interacting proteins. SIAH1 has been suggested as part of a novel regulatory loop controlling the level of OCA-B protein in B cells. B-cell receptor cross-linking results in a degradation of OCA-B, which further results in a reduction of transcriptional activation of OCA-B-dependent reporters (2, 3).
Synonyms: POU2AF1, OBF1, POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1,OCT-binding factor 1,BOB-1,OCA-B
Molecular Weight 35 kDA
Gene ID 5450
UniProt Q16633
Research Area Ubiquitin-related antibodies, Protein Modifications, Cancer, Transcription Factors, Signaling, Chromatin, Cell Structure
Application Notes IHC: = 1:100-250, WB: = 1:5000-50000
Comment

Background: OCA-B is a lymphoid-specific transcriptional coactivator which interacts with the transcription factors Oct-1 and Oct-2 (1). It has been shown to be critical for development of a normal immune response, where it mediates octamer-dependent transcriptional activity in B lymphocytes. It is also critically involved in the formation of germinal centers in secondary lymphoid organs. OCA-B levels have been observed to be massively upregulated in germinal center B cells, as compared with resting B cells. SIAH1 and SIAH2, known regulators of protein stability, have been identified as two related OCA-B interacting proteins. SIAH1 has been suggested as part of a novel regulatory loop controlling the level of OCA-B protein in B cells. B-cell receptor cross-linking results in a degradation of OCA-B, which further results in a reduction of transcriptional activation of OCA-B-dependent reporters (2, 3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment OCA-B Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Tiedt, Bartholdy, Matthias et al.: "The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1." in: The EMBO journal, Vol. 20, Issue 15, pp. 4143-52, 2001 (PubMed).

Bartholdy, Du Roure, Bordon et al.: "The Ets factor Spi-B is a direct critical target of the coactivator OBF-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 31, pp. 11665-70, 2006 (PubMed).

Validation Images
back to top