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Details for Product No. ABIN650151

POU Class 2 Associating Factor 1 (POU2AF1) antibody

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Antigen
Synonyms BOB1, OBF1, OCAB, OBF-1, BOB.1, Bob-1, Bob1, OBF.1, OCA-B, BOB-1
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Pubmed 2 references available
Catalog no. ABIN650151
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic peptide corresponding to residues in human OCA-B was used as an immunogen.
Alternative Name OCA-B
Background OCA-B is a lymphoid-specific transcriptional coactivator which interacts with the transcription factors Oct-1 and Oct-2 (1). It has been shown to be critical for development of a normal immune response, where it mediates octamer-dependent transcriptional activity in B lymphocytes. It is also critically involved in the formation of germinal centers in secondary lymphoid organs. OCA-B levels have been observed to be massively upregulated in germinal center B cells, as compared with resting B cells. SIAH1 and SIAH2, known regulators of protein stability, have been identified as two related OCA-B interacting proteins. SIAH1 has been suggested as part of a novel regulatory loop controlling the level of OCA-B protein in B cells. B-cell receptor cross-linking results in a degradation of OCA-B, which further results in a reduction of transcriptional activation of OCA-B-dependent reporters (2, 3).
Synonyms: POU2AF1, OBF1, POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1,OCT-binding factor 1,BOB-1,OCA-B
Molecular Weight 35 kDA
Gene ID 5450
UniProt Q16633
Research Area Ubiquitin-related antibodies, Protein Modifications, Cancer, Transcription Factors, Signaling, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:5000~50000 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250
Comment

Background: OCA-B is a lymphoid-specific transcriptional coactivator which interacts with the transcription factors Oct-1 and Oct-2 (1). It has been shown to be critical for development of a normal immune response, where it mediates octamer-dependent transcriptional activity in B lymphocytes. It is also critically involved in the formation of germinal centers in secondary lymphoid organs. OCA-B levels have been observed to be massively upregulated in germinal center B cells, as compared with resting B cells. SIAH1 and SIAH2, known regulators of protein stability, have been identified as two related OCA-B interacting proteins. SIAH1 has been suggested as part of a novel regulatory loop controlling the level of OCA-B protein in B cells. B-cell receptor cross-linking results in a degradation of OCA-B, which further results in a reduction of transcriptional activation of OCA-B-dependent reporters (2, 3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment OCA-B Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Tiedt, Bartholdy, Matthias et al.: "The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1." in: The EMBO journal, Vol. 20, Issue 15, pp. 4143-52, 2001 (PubMed).

Bartholdy, Du Roure, Bordon et al.: "The Ets factor Spi-B is a direct critical target of the coactivator OBF-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 31, pp. 11665-70, 2006 (PubMed).

Alternatives for antigen "POU Class 2 Associating Factor 1 (POU2AF1)", type "Antibodies"
Hosts (57), (13), (6)
Reactivities (71), (34), (31), (27), (26), (24), (12)
Applications (41), (34), (20), (19), (6), (5), (2), (2), (2), (1), (1), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (20), (4), (2), (2), (1), (1)
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