POU Class 2 Associating Factor 1 (POU2AF1) antibody

Antigen: POU Class 2 Associating Factor 1 (POU2AF1)

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650152

Additional Information

Alternative name: OCA-B

Gene ID: 5081

UniProt: Q16633

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human OCA-B was used as an immunogen.

Molecular Weight: 35 kDA

Comments:

Background: OCA-B is a lymphoid-specific transcriptional coactivator which interacts with the transcription factors Oct-1 and Oct-2 (1). It has been shown to be critical for development of a normal immune response, where it mediates octamer-dependent transcriptional activity in B lymphocytes. It is also critically involved in the formation of germinal centers in secondary lymphoid organs. OCA-B levels have been observed to be massively upregulated in germinal center B cells, as compared with resting B cells. SIAH1 and SIAH2, known regulators of protein stability, have been identified as two related OCA-B interacting proteins. SIAH1 has been suggested as part of a novel regulatory loop controlling the level of OCA-B protein in B cells. B-cell receptor cross-linking results in a degradation of OCA-B, which further results in a reduction of transcriptional activation of OCA-B-dependent reporters (2, 3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:2000~10000 IP: ~~ 1:50 FC: ~~ 1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: OCA-B Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cancer, Transcription Factors, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Tiedt, Bartholdy, Matthias et al.: "The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1." in: The EMBO journal, Vol. 20, Issue 15, pp. 4143-52, 2001 (PubMed).

Boehm, He, Greiner et al.: "Regulation of BOB.1/OBF.1 stability by SIAH." in: The EMBO journal, Vol. 20, Issue 15, pp. 4153-62, 2001 (PubMed).

Bartholdy, Du Roure, Bordon et al.: "The Ets factor Spi-B is a direct critical target of the coactivator OBF-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 31, pp. 11665-70, 2006 (PubMed).