Orthodenticle Homeobox 2 (OTX2) antibody

Antigen: Orthodenticle Homeobox 2 (OTX2)

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN650157

Additional Information

Alternative name: OTX2

Gene ID: 5015

UniProt: P32243

Characteristics: Rabbit Monoclonal IgG

Specificity: A synthetic peptide corresponding to residues in human OTX2 was used as an immunogen.

Molecular Weight: 32 kDA

Comments:

Background: Homeobox protein OTX2 is a member of the bicoid sub-family of homeodomain-containing transcription factors. It acts as a transcription factor and is reported to play a role in brain and sensory organ development (1), more specifically, forebrain and eye development (2). The OTX2 is required to determine mesencephalic versus metencephalic (cerebellum/pons) territory during embryogenesis. This function of OTX2 primarily involves positioning and maintaining the mid-hindbrain organizer at the border between midbrain and anterior hindbrain (3). OTX2 is expressed during development in the neuroepithelium of most of the forebrain and midbrain, including the eye domain. In mice, complete elimination of the OTX2 function results in the absence of the forebrain and embryonic lethality (4). Defects in OTX2 are the caused of microphthalmia syndromic type 5, a clinically heterogeneous disorder of the eye formation (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: OTX2 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Vernay, Koch, Vaccarino et al.: "Otx2 regulates subtype specification and neurogenesis in the midbrain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 19, pp. 4856-67, 2005 (PubMed).

Chatelain, Fossat, Brun et al.: "Molecular dissection reveals decreased activity and not dominant negative effect in human OTX2 mutants." in: Journal of molecular medicine (Berlin, Germany), Vol. 84, Issue 7, pp. 604-15, 2006 (PubMed).

Pruitt, Tatusova, Maglott: "NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins." in: Nucleic acids research, Vol. 35, Issue Database issue, pp. D61-5, 2007 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).

Henderson, Williamson, Kennedy et al.: "A rare de novo nonsense mutation in OTX2 causes early onset retinal dystrophy and pituitary dysfunction." in: Molecular vision, Vol. 15, pp. 2442-7, 2009 (PubMed).