|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (pTyr410) antibody
|Synonyms||CAS, CAS1, CASS1, CRKAS, P130Cas, Cas, Crkas, AI385681, P130CAS, MGC68466, cas, p130cas, BCAR1, bcar1, fc17f11, MGC175192, wu:fc17f11|
Alternatives Western Blotting (WB), Immunoprecipitation (IP)
|3 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues surrounding Tyrosine 410 of human p130Cas was used as an immunogen.|
|Molecular Weight||130 kDA|
Background: Breast cancer anti-estrogen resistance protein 1 (p130Cas) is a docking protein that plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion (1). It has defined function in cardiovascular development, actin filament assembly and Src-induced transformation (2). p130Cas is highly phosphorylated at tyrosines during transformation by v-Src, as well as by v-Crk, forming stable complexes with these oncoproteins. Cytoplasmic p130Cas has been shown to move to the membrane upon tyrosine phosphorylation. p130Cas is a common cellular target of phosphorylation signal via v-Crk and v-Src oncoproteins, and its unique structure indicates its possible role in assembling signals from multiple SH2-containing molecules (3). It is implicated in induction of cell migration, and over expression of p130Cas confers anti-estrogen resistance on breast cancer cells (1).
|Application Notes||The suggested dilution is: WB: ~~ 1:2500~5000 IP: ~~ 1:50|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||p130Cas Antibody Phospho (pY410) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
Ruest, Shin, Polte et al.: "Mechanisms of CAS substrate domain tyrosine phosphorylation by FAK and Src." in: Molecular and cellular biology, Vol. 21, Issue 22, pp. 7641-52, 2001 (PubMed).
Bouton, Riggins, Bruce-Staskal: "Functions of the adapter protein Cas: signal convergence and the determination of cellular responses." in: Oncogene, Vol. 20, Issue 44, pp. 6448-58, 2001 (PubMed).
"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).