Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (pTyr410) antibody

Details for Product No. ABIN650168
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Antigen
Synonyms CAS, CAS1, CASS1, CRKAS, P130Cas, Cas, Crkas, AI385681, MGC68466, cas, p130cas, BCAR1, fc17f11, wu:fc17f11, zgc:175192
Epitope
pTyr410
(27), (17), (16), (16), (12), (6), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(150), (85), (82), (16), (12), (4), (1)
Host
Rabbit
(133), (22), (1), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application
Western Blotting (WB)
(103), (65), (50), (42), (34), (14), (14), (7), (4), (3), (2)
Pubmed 3 references available
Catalog no. ABIN650168
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
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Specificity A synthetic peptide corresponding to residues surrounding Tyrosine 410 of human p130Cas was used as an immunogen.
Alternative Name p130cas
Background Breast cancer anti-estrogen resistance protein 1 (p130Cas) is a docking protein that plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion (1). It has defined function in cardiovascular development, actin filament assembly and Src-induced transformation (2). p130Cas is highly phosphorylated at tyrosines during transformation by v-Src, as well as by v-Crk, forming stable complexes with these oncoproteins. Cytoplasmic p130Cas has been shown to move to the membrane upon tyrosine phosphorylation. p130Cas is a common cellular target of phosphorylation signal via v-Crk and v-Src oncoproteins, and its unique structure indicates its possible role in assembling signals from multiple SH2-containing molecules (3). It is implicated in induction of cell migration, and over expression of p130Cas confers anti-estrogen resistance on breast cancer cells (1).
Molecular Weight 130 kDA
Gene ID 112398
UniProt P56945
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: = 1:2500-5000
Comment

Background: Breast cancer anti-estrogen resistance protein 1 (p130Cas) is a docking protein that plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion (1). It has defined function in cardiovascular development, actin filament assembly and Src-induced transformation (2). p130Cas is highly phosphorylated at tyrosines during transformation by v-Src, as well as by v-Crk, forming stable complexes with these oncoproteins. Cytoplasmic p130Cas has been shown to move to the membrane upon tyrosine phosphorylation. p130Cas is a common cellular target of phosphorylation signal via v-Crk and v-Src oncoproteins, and its unique structure indicates its possible role in assembling signals from multiple SH2-containing molecules (3). It is implicated in induction of cell migration, and over expression of p130Cas confers anti-estrogen resistance on breast cancer cells (1).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment p130Cas Antibody Phospho (pY410) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Ruest, Shin, Polte et al.: "Mechanisms of CAS substrate domain tyrosine phosphorylation by FAK and Src." in: Molecular and cellular biology, Vol. 21, Issue 22, pp. 7641-52, 2001 (PubMed).

Bouton, Riggins, Bruce-Staskal: "Functions of the adapter protein Cas: signal convergence and the determination of cellular responses." in: Oncogene, Vol. 20, Issue 44, pp. 6448-58, 2001 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).

Hosts (133), (22), (1), (1)
Reactivities (150), (85), (82), (16), (12), (4), (1)
Applications (103), (65), (50), (42), (34), (14), (14), (7), (4), (3), (2)
Conjugates (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Epitopes (27), (17), (16), (16), (12), (6), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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