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Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (pTyr410) antibody

Antigen

Breast Cancer Anti-Estrogen Resistance 1 (BCAR1)
Synonyms CAS, CAS1, CASS1, CRKAS, P130Cas, Cas, Crkas, AI385681, P130CAS, MGC68466, cas, p130cas, BCAR1, bcar1, fc17f11, MGC175192, wu:fc17f11
Binding Site
Alternatives

pTyr410
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunoprecipitation (IP)
3 references available
Certificates ISO 9001:2008
Catalog no. ABIN650168
Quantity 100ul
Price 507.14 $   Plus shipping costs $45.00
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Additional Information
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Alternative name p130cas
Gene ID 112398
UniProt P56945
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues surrounding Tyrosine 410 of human p130Cas was used as an immunogen.
Molecular Weight 130 kDA
Comments

Background: Breast cancer anti-estrogen resistance protein 1 (p130Cas) is a docking protein that plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion (1). It has defined function in cardiovascular development, actin filament assembly and Src-induced transformation (2). p130Cas is highly phosphorylated at tyrosines during transformation by v-Src, as well as by v-Crk, forming stable complexes with these oncoproteins. Cytoplasmic p130Cas has been shown to move to the membrane upon tyrosine phosphorylation. p130Cas is a common cellular target of phosphorylation signal via v-Crk and v-Src oncoproteins, and its unique structure indicates its possible role in assembling signals from multiple SH2-containing molecules (3). It is implicated in induction of cell migration, and over expression of p130Cas confers anti-estrogen resistance on breast cancer cells (1).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:2500~5000 IP: ~~ 1:50
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage p130Cas Antibody Phospho (pY410) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Ruest, Shin, Polte et al.: "Mechanisms of CAS substrate domain tyrosine phosphorylation by FAK and Src." in: Molecular and cellular biology, Vol. 21, Issue 22, pp. 7641-52, 2001 (PubMed).

Bouton, Riggins, Bruce-Staskal: "Functions of the adapter protein Cas: signal convergence and the determination of cellular responses." in: Oncogene, Vol. 20, Issue 44, pp. 6448-58, 2001 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Breast Cancer Anti-Estrogen Resistance 1 (BCAR1)", type "Antibodies"
Hosts Rabbit (52), Mouse (12), Sheep (1)
Reactivities Human (60), Rat (Rattus) (49), Mouse (Murine) (42), Dog (Canine) (23), Cow (Bovine) (19), Horse (Equine) (5), Monkey (4), Cat (Feline) (1), Chicken (1)
Applications Western Blotting (WB) (35), Immunofluorescence (IF) (33), ELISA (21), Immunohistochemistry (IHC) (17), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (16), Immunoprecipitation (IP) (10), Flow Cytometry (FACS) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (4), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes C-Term (5), pTyr165 (5), pTyr410 (5), AA 949-968 (2), Internal Region (2), AA 66-412 (1), AA 670-896 (1), AA 691-799 (1), C-Term,pSer639 (1), Center, Tyr224 (1), pSer2481 (1), pTyr234 (1), pTyr249 (1)