GTPase Kras (pSer145) antibody

Details for Product No. ABIN650172
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Antigen
Synonyms
Kras2, c-Ki-ras, p21, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2, SIIR, pp21, 0610011M09Rik, BC011290, P21, AI929937, K-ras, Ki-ras, Kras-2, p21B, ras, wu: ... show more
Kras2, c-Ki-ras, p21, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2, SIIR, pp21, 0610011M09Rik, BC011290, P21, AI929937, K-ras, Ki-ras, Kras-2, p21B, ras, wu:fa04e08, wu:fc14b12, wu:fc23g10, wu:fj89d12, zgc:85725 show less
Epitope
pSer145
(10), (5), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(97), (40), (39), (13), (2), (1), (1), (1), (1)
Host
Rabbit
(82), (22)
Clonality
Monoclonal
Conjugate
Un-conjugated
(6), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(80), (47), (23), (20), (12), (10), (9), (4), (3), (1)
Pubmed 3 references available
Quantity 100 μL
Options
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Catalog No. ABIN650172
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Immunogen A phospho-specific peptide corresponding to residues surrounding Serine 145 of human p21 was used as an immunogen.
Specificity A phospho-specific peptide corresponding to residues surrounding Serine 145 of human p21 was used as an immunogen.
Alternative Name p21
Background Cyclin-dependent kinase inhibitor 1 (CDK-interacting protein 1, p21, CDKN1A, WAF1) plays a critical role in the cellular response to DNA damage, and its overexpression results in cell cycle arrest (1). p21 binds to and inhibits CDK activity, preventing phosphorylation of critical CDK substrates and blocking cell cycle progression. p21 is suggested to possibly be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage (2). Endogenous cellular p21 is shown to be completely acetylated at its amino terminus and is therefore not a substrate for N-ubiquitylation (3). Phosphorylation of Threonine 144 by Akt or of Serine 145 by PKC impairs p21 binding to PCNA (2).
Synonyms: CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1, Cyclin-dependent kinase inhibitor 1, p21,CDK-interacting protein 1,Melanoma differentiation-associated protein 6
Molecular Weight 21 kDA
Gene ID 1026
UniProt P38936
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes IHC: = 1:100-250, WB: = 1:1000-5000
Comment

Background: Cyclin-dependent kinase inhibitor 1 (CDK-interacting protein 1, p21, CDKN1A, WAF1) plays a critical role in the cellular response to DNA damage, and its overexpression results in cell cycle arrest (1). p21 binds to and inhibits CDK activity, preventing phosphorylation of critical CDK substrates and blocking cell cycle progression. p21 is suggested to possibly be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage (2). Endogenous cellular p21 is shown to be completely acetylated at its amino terminus and is therefore not a substrate for N-ubiquitylation (3). Phosphorylation of Threonine 144 by Akt or of Serine 145 by PKC impairs p21 binding to PCNA (2).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment p21 Antibody Phospho (pS145) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Bendjennat, Boulaire, Jascur et al.: "UV irradiation triggers ubiquitin-dependent degradation of p21(WAF1) to promote DNA repair." in: Cell, Vol. 114, Issue 5, pp. 599-610, 2003 (PubMed).

Chen, Chi, Bloecher et al.: "N-acetylation and ubiquitin-independent proteasomal degradation of p21(Cip1)." in: Molecular cell, Vol. 16, Issue 5, pp. 839-47, 2004 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).

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