GTPase Kras (pSer145) antibody

Antigen: GTPase Kras

Synonyms: NS, NS3, KRAS1, KRAS2, RASK2, KI-RAS, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, ras, p21B, K-ras, Kras2, Ki-ras, Kras-2, MGC7141, AI929937

Binding Site: pSer145

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN650172

Additional Information

Alternative name: p21

Gene ID: 1026

UniProt: P38936

Immunogen: A phospho-specific peptide corresponding to residues surrounding Serine 145 of human p21 was used as an immunogen.

Description: Other names: CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1, Cyclin-dependent kinase inhibitor 1, p21,CDK-interacting protein 1,Melanoma differentiation-associated protein 6

Characteristics: Rabbit Monoclonal Antibody

Specificity: A phospho-specific peptide corresponding to residues surrounding Serine 145 of human p21 was used as an immunogen.

Molecular Weight: 21 kDA

Comments:

Background: Cyclin-dependent kinase inhibitor 1 (CDK-interacting protein 1, p21, CDKN1A, WAF1) plays a critical role in the cellular response to DNA damage, and its overexpression results in cell cycle arrest (1). p21 binds to and inhibits CDK activity, preventing phosphorylation of critical CDK substrates and blocking cell cycle progression. p21 is suggested to possibly be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage (2). Endogenous cellular p21 is shown to be completely acetylated at its amino terminus and is therefore not a substrate for N-ubiquitylation (3). Phosphorylation of Threonine 144 by Akt or of Serine 145 by PKC impairs p21 binding to PCNA (2).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~5000 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: p21 Antibody Phospho (pS145) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Bendjennat, Boulaire, Jascur et al.: "UV irradiation triggers ubiquitin-dependent degradation of p21(WAF1) to promote DNA repair." in: Cell, Vol. 114, Issue 5, pp. 599-610, 2003 (PubMed).

Chen, Chi, Bloecher et al.: "N-acetylation and ubiquitin-independent proteasomal degradation of p21(Cip1)." in: Molecular cell, Vol. 16, Issue 5, pp. 839-47, 2004 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).