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CDKN1B antibody (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1))

Details for Product anti-CDKN1B Antibody No. ABIN650175, Supplier: Login to see New
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Antigen
Alternatives
Reactivity
Rat (Rattus)
(600), (211), (148), (21), (21), (16), (10), (7), (6), (5), (3), (2), (2), (2), (2), (1), (1)
Host
Rabbit
(512), (105), (37), (4), (2), (1)
Clonality
Monoclonal
Conjugate
This CDKN1B antibody is un-conjugated
(31), (31), (27), (25), (25), (25), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(449), (305), (184), (103), (102), (86), (51), (30), (29), (16), (14), (9), (3), (1), (1), (1)
Pubmed 3 references available
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Quantity 100 μL
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Immunogen A recombinant protein in mouse p27/Kip1was used as an immunogen.
Specificity A recombinant protein in mouse p27/Kip1was used as an immunogen.
Alternative Name p27/Kip1 (CDKN1B Antibody Abstract)
Background P27/Kip1 is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27/Kip1 binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27/Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27/Kip1 (2), while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).
Synonyms: Cdkn1b, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27,p27Kip1
Molecular Weight 27 kDA
Gene ID 1081
UniProt P46414
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes IHC: = 1:100-250, WB: = 1:1000-5000
Comment

Background: p27/Kip1 is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27/Kip1 binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27/Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27/Kip1 (2), while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment p27/Kip1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Rodier, Montagnoli, Di Marcotullio et al.: "p27 cytoplasmic localization is regulated by phosphorylation on Ser10 and is not a prerequisite for its proteolysis." in: The EMBO journal, Vol. 20, Issue 23, pp. 6672-82, 2001 (PubMed).

Carrano, Eytan, Hershko et al.: "SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27." in: Nature cell biology, Vol. 1, Issue 4, pp. 193-9, 1999 (PubMed).

Polyak, Lee, Erdjument-Bromage et al.: "Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals." in: Cell, Vol. 78, Issue 1, pp. 59-66, 1994 (PubMed).

Catalog No. ABIN650175

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