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CDKN1B antibody (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1))

Details for Product anti-CDKN1B Antibody No. ABIN650176, Supplier: Login to see New
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Antigen
Alternatives
Reactivity
Human
(600), (211), (148), (21), (21), (16), (10), (7), (6), (5), (3), (2), (2), (2), (2), (1), (1)
Host
Rabbit
(512), (105), (37), (4), (2), (1)
Clonality
Monoclonal
Conjugate
This CDKN1B antibody is un-conjugated
(31), (31), (27), (25), (25), (25), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(449), (305), (184), (103), (102), (86), (51), (30), (29), (16), (14), (9), (3), (1), (1), (1)
Pubmed 4 references available
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Quantity 100 μL
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Immunogen A synthetic peptide corresponding to residues in human p27/Kip1 was used as an immunogen.
Specificity A synthetic peptide corresponding to residues in human p27/Kip1 was used as an immunogen.
Alternative Name p27/Kip1 (CDKN1B Antibody Abstract)
Background P27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. It is an inhibitor of cyclinE-Cdk2 complex, cyclinA-Cdk2 and cyclinD1-Cdk4 (1), and a positive regulator of cyclin D-dependent kinases such as Cdk4 (2). It plays a pivotal role in the control of cell proliferation and the loss of p27/Kip1 function may lead to carcinogenesis (4). The function of p27/Kip1 is regulated by phosphorylation and degradation events. Phosphorylation by hKIS on Serine 10 signals the nuclear export to the cytoplasm (3), while phosphorylation by Cdk2 on Threonine 187 results in ubiquitylation and degradation of p27/Kip1 (2).
Molecular Weight 27 kDA
Gene ID 5130
UniProt P46414
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes The suggested dilution is: WB: = 1:1000-10000
Comment

Background: p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. It is an inhibitor of cyclinE-Cdk2 complex, cyclinA-Cdk2 and cyclinD1-Cdk4 (1), and a positive regulator of cyclin D-dependent kinases such as Cdk4 (2). It plays a pivotal role in the control of cell proliferation and the loss of p27/Kip1 function may lead to carcinogenesis (4). The function of p27/Kip1 is regulated by phosphorylation and degradation events. Phosphorylation by hKIS on Serine 10 signals the nuclear export to the cytoplasm (3), while phosphorylation by Cdk2 on Threonine 187 results in ubiquitylation and degradation of p27/Kip1 (2).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment p27/Kip1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications "The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).

Ishida, Hara, Kamura et al.: "Phosphorylation of p27Kip1 on serine 10 is required for its binding to CRM1 and nuclear export." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 14355-8, 2002 (PubMed).

Carrano, Eytan, Hershko et al.: "SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27." in: Nature cell biology, Vol. 1, Issue 4, pp. 193-9, 1999 (PubMed).

Polyak, Lee, Erdjument-Bromage et al.: "Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals." in: Cell, Vol. 78, Issue 1, pp. 59-66, 1994 (PubMed).

Catalog No. ABIN650176

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