Cyclin-Dependent Kinase Inhibitor 1B (CDKN1B) (C-Term) antibody

Antigen: Cyclin-Dependent Kinase Inhibitor 1B (CDKN1B)

Synonyms: KIP1, MEN4, CDKN4, MEN1B, P27KIP1, p27, Rpn4, MGC8644, Kip1, Cdki1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650177

Additional Information

Alternative name: p27/Kip1

Gene ID: 1027

UniProt: P46527

Immunogen: A synthetic peptide corresponding to residues in the C-term of human p27(Kip1) was used as immunogen

Description: Other names: CDKN1B, KIP1, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27,p27Kip1

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in the C-term of human p27(Kip1) was used as immunogen

Molecular Weight: 27 kDA

Comments:

Background: p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2), while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500 FC: ~~ 1:40 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: p27/Kip1 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Publications

Product: Polyak, Lee, Erdjument-Bromage et al.: "Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals." in: Cell, Vol. 78, Issue 1, pp. 59-66, 1994 (PubMed).

Carrano, Eytan, Hershko et al.: "SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27." in: Nature cell biology, Vol. 1, Issue 4, pp. 193-9, 1999 (PubMed).

Rodier, Montagnoli, Di Marcotullio et al.: "p27 cytoplasmic localization is regulated by phosphorylation on Ser10 and is not a prerequisite for its proteolysis." in: The EMBO journal, Vol. 20, Issue 23, pp. 6672-82, 2001 (PubMed).