Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pSer100) antibody

Details for Product No. ABIN650178
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Antigen
Synonyms
CDKN1B, CIB, CIBP, KIP1, PRKDCIP, SIP2-28, p27Kip1, AA408329, AI843786, Kip1, p27, CDKN4, Cdki1b, P27KIP1, MEN1B, MEN4, cdkn1bl, cdkn1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, FAM14D, ISG12, IS ... show more
CDKN1B, CIB, CIBP, KIP1, PRKDCIP, SIP2-28, p27Kip1, AA408329, AI843786, Kip1, p27, CDKN4, Cdki1b, P27KIP1, MEN1B, MEN4, cdkn1bl, cdkn1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, FAM14D, ISG12, ISG12A, P27, 1500011J20Rik, 2610202L11Rik, Bridge-1 show less
Epitope
pSer100
(69), (52), (35), (34), (28), (21), (13), (11), (10), (10), (9), (9), (9), (7), (7), (6), (6), (6), (6), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(412), (182), (144), (81), (61), (54), (36), (13), (8), (3), (3), (1), (1), (1), (1)
Host
Rabbit
(383), (64), (14), (1), (1), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(21), (19), (17), (11), (11), (11), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(300), (187), (91), (89), (60), (60), (51), (28), (13), (10), (6), (6), (1)
Pubmed 2 references available
Quantity 100 µL
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Catalog No. ABIN650178
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Immunogen A phospho specific peptide corresponding to residues surrounding Serine 10 of human p27 was used as an immunogen. This antibody detects p27 phosphorylated at Serine 10.
Specificity A phospho specific peptide corresponding to residues surrounding Serine 10 of human p27 was used as an immunogen. This antibody detects p27 phosphorylated at Serine 10.
Alternative Name p27/Kip1
Background P27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2), while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).
Synonyms: CDKN1B, KIP1, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27,p27Kip1
Molecular Weight 27 kDA
Gene ID 1027
UniProt P46527
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cancer, Cell Structure
Application Notes IHC: = 1:100, WB: = 1:1000-10000
Comment

Background: p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2), while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment p27/Kip1 Antibody Phospho (pS10) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Polyak, Lee, Erdjument-Bromage et al.: "Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals." in: Cell, Vol. 78, Issue 1, pp. 59-66, 1994 (PubMed).

Rodier, Montagnoli, Di Marcotullio et al.: "p27 cytoplasmic localization is regulated by phosphorylation on Ser10 and is not a prerequisite for its proteolysis." in: The EMBO journal, Vol. 20, Issue 23, pp. 6672-82, 2001 (PubMed).

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