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Details for Product No. ABIN650178

Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pSer100) antibody

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Antigen
Synonyms
P27, ISG12, FAM14D, ISG12A, KIP1, MEN4, CDKN4, MEN1B, P27KIP1, AA408329, AI843786, Kip1, p27, p27Kip1, 1500011J20Rik, 2610202L11Rik, Bridge-1, Cdki1b, cdkn1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18 ... show more
Epitope
»Alternatives pSer100
Reactivity
»Alternatives Human, Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Pubmed 2 references available
Catalog no. ABIN650178
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
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Immunogen A phospho specific peptide corresponding to residues surrounding Serine 10 of human p27 was used as an immunogen. This antibody detects p27 phosphorylated at Serine 10.
Specificity A phospho specific peptide corresponding to residues surrounding Serine 10 of human p27 was used as an immunogen. This antibody detects p27 phosphorylated at Serine 10.
Alternative Name p27/Kip1
Background P27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2), while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).
Synonyms: CDKN1B, KIP1, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27,p27Kip1
Molecular Weight 27 kDA
Gene ID 1027
UniProt P46527
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cancer, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:30 IHC: ~~ 1:100
Comment

Background: p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2), while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment p27/Kip1 Antibody Phospho (pS10) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Polyak, Lee, Erdjument-Bromage et al.: "Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals." in: Cell, Vol. 78, Issue 1, pp. 59-66, 1994 (PubMed).

Rodier, Montagnoli, Di Marcotullio et al.: "p27 cytoplasmic localization is regulated by phosphorylation on Ser10 and is not a prerequisite for its proteolysis." in: The EMBO journal, Vol. 20, Issue 23, pp. 6672-82, 2001 (PubMed).

Alternatives for antigen "Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B)", type "Antibodies"
Hosts (301), (64), (16), (2), (1)
Reactivities (343), (172), (145), (83), (61), (54), (36), (14), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Applications (260), (134), (100), (68), (60), (54), (29), (18), (13), (11), (8), (5), (2), (1)
Conjugates (13), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2)
Epitopes (58), (42), (26), (24), (21), (20), (12), (8), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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