Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180, pTyr182) antibody

Antigen: Mitogen-Activated Protein Kinase 14 (MAPK14)

Synonyms: RK, p38, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, Hog, CRK1, CSBP, Exip, Csbp1, Csbp2, Prkm14, Prkm15, Sapk2A, p38Hog, p38alpha, MGC105413, p38b, zp38b, MAPK14, MGC142910, 186F5S, BG:DS00797.3, D-p38, D-p38b, Dm p38b, Dp38, ESTS:186F5S, Mpk34C, anon-sts23, p38 MAPK, p38 beta, p38B, p38beta, DmelCG7393, CG7393

Binding Site: pThr180, pTyr182

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650181

Additional Information

Alternative name: p38 MAPK

Gene ID: 1432

UniProt: Q16539

Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr180 and Tyr182 of human p38 MAPK was used as immunogen. The antibody only detects p38 MAPK phosphorylated on Threonine 180 and Tyrosine 182.

Description: Other names: MAPK14, CSBP, CSBP1, CSBP2, CSPB1, MXI2, Mitogen-activated protein kinase 14, MAX-interacting protein 2,MAP kinase MXI2,SAPK2A

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic phospho-peptide corresponding to residues surrounding Thr180 and Tyr182 of human p38 MAPK was used as immunogen. The antibody only detects p38 MAPK phosphorylated on Threonine 180 and Tyrosine 182.

Molecular Weight: 42 kDA

Comments:

Background: p38 MAP kinase is a mitogen-activated protein kinase that functions in various intracellular signaling cascades controlling cellular stress responses. p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-3). MKK3 and SEK activate p38 MAP kinase by phosphorylation at adjacent threonine and tyrosine residues, Thr180 and Tyr182 (3,4). Activated p38 MAP kinase phosphorylates numerous downstream targets, including transcription factor ATF-2 (4).

Synonyms: RK, p38, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, Hog, CRK1, CSBP, Exip, Csbp1, Csbp2, Prkm14, Prkm15, Sapk2A, p38Hog, p38alpha, MGC105413, p38b, zp38b, MAPK14, MGC142910, 186F5S, BG:DS00797.3, D-p38, D-p38b, Dm p38b, Dp38, ESTS:186F5S, Mpk34C, anon-sts23, p38 MAPK, p38 beta, p38B, p38beta, DmelCG7393, CG7393

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~5000 IP: ~~ 1:10 ICC: ~~ 1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: p38 MAPK Antibody Phospho (pT180/pY182) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Raingeaud, Gupta, Rogers et al.: "Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine." in: The Journal of biological chemistry, Vol. 270, Issue 13, pp. 7420-6, 1995 (PubMed).

Han, Richter, Li et al.: "Molecular cloning of human p38 MAP kinase." in: Biochimica et biophysica acta, Vol. 1265, Issue 2-3, pp. 224-7, 1995 (PubMed).

Han, Lee, Bibbs et al.: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." in: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, 1994 (PubMed).