Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (C-Term) antibody

Details for Product No. ABIN650182
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Antigen
Synonyms p40phox, AI451400, NCF, P40PHOX, SH3PXD4
Epitope
C-Term
(18), (9), (8), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(80), (44), (33), (30), (24), (14), (12), (2)
Host
Rabbit
(68), (8), (7)
Clonality
Monoclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(47), (27), (22), (20), (17), (5), (3), (2), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 100 μL
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Catalog No. ABIN650182
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Immunogen A synthetic peptide corresponding to residues near the C-terminus of human p40 was used as an immunogen.
Specificity A synthetic peptide corresponding to residues near the C-terminus of human p40 was used as an immunogen.
Alternative Name p40 (phox)
Background The NADPH oxidase generates superoxide in phagocytic cells. It is important for immunity and its deficiency leads to chronic granulomatous disease (CGD) (1). p40-phox is a newly isolated cytosolic component of the nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase that copurifies with p67-phox. Preliminary evidence indicates that it is a component of the cytosolic complex (2). Maximal activation of NADPH oxidase requires formation of a complex between the p40-phox and p67-phox subunits via association of their PB1 domains. The crystal structure of the p40-phox/p67phox PB1 heterodimer reveals that both domains have a beta grasp topology and that they bind in a front-to-back arrangement through conserved electrostatic interactions between an acidic OPCA motif on p40-phox and basic residues in p67-phox (3).
Synonyms: NCF4, SH3PXD4, Neutrophil cytosol factor 4, SH3 and PX domain-containing protein 4
Molecular Weight 40 kDA
Gene ID 4689
UniProt Q15080
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes IHC: = 1:100-250, WB: = 1:25000-50000
Comment

Background: The NADPH oxidase generates superoxide in phagocytic cells. It is important for immunity and its deficiency leads to chronic granulomatous disease (CGD) (1). p40-phox is a newly isolated cytosolic component of the nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase that copurifies with p67-phox. Preliminary evidence indicates that it is a component of the cytosolic complex (2). Maximal activation of NADPH oxidase requires formation of a complex between the p40-phox and p67-phox subunits via association of their PB1 domains. The crystal structure of the p40-phox/p67phox PB1 heterodimer reveals that both domains have a beta grasp topology and that they bind in a front-to-back arrangement through conserved electrostatic interactions between an acidic OPCA motif on p40-phox and basic residues in p67-phox (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment p40 (phox) Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Wientjes, Hsuan, Totty et al.: "p40phox, a third cytosolic component of the activation complex of the NADPH oxidase to contain src homology 3 domains." in: The Biochemical journal, Vol. 296 ( Pt 3), pp. 557-61, 1994 (PubMed).

Zhan, Vazquez, Zhan et al.: "Genomic structure, chromosomal localization, start of transcription, and tissue expression of the human p40-phox, a new component of the nicotinamide adenine dinucleotide phosphate-oxidase complex." in: Blood, Vol. 88, Issue 7, pp. 2714-21, 1996 (PubMed).

Wilson, Gill, Perisic et al.: "PB1 domain-mediated heterodimerization in NADPH oxidase and signaling complexes of atypical protein kinase C with Par6 and p62." in: Molecular cell, Vol. 12, Issue 1, pp. 39-50, 2003 (PubMed).

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