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Tumor Protein P53 (TP53) antibody

Antigen

Tumor Protein P53 (TP53)
Synonyms p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
4 references available
Certificates ISO 9001:2008
Catalog no. ABIN650183
Quantity 100ul
Price 450.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name p53
Gene ID 7157
UniProt P04637
Immunogen A synthetic peptide corresponding to residues following the transcription activation domain of human p53 was used as immunogen. The antibody should recognize human wild-type and mutant p53.
Description Other names: TP53, P53, Cellular tumor antigen p53, Tumor suppressor p53,Phosphoprotein p53,Antigen NY-CO-13
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues following the transcription activation domain of human p53 was used as immunogen. The antibody should recognize human wild-type and mutant p53.
Molecular Weight 53 kDA
Comments

Background: p53 acts as both a tumor-suppressor and transcription factor that, upon activation by DNA damage and other cellular stress signals, leads to the transcription of genes triggering cell-cycle arrest, apoptosis, and DNA repair (1,2). The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers (3). p53 is frequently mutated or inactivated in about 60% of cancers (4,5).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:10000~50000 ICC: ~~ 1:50~100 IHC: ~~ 1:100
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage p53 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Research Area Translation Factors, Protein Modifications, Signaling, Cell Structure, Ubiquitin-related antibodies, Apoptosis/Necrosis, Phospho-specific antibodies, Cancer, Transcription Factors
Restrictions For Research Use only

Publications
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Product Vogelstein: "Cancer. A deadly inheritance." in: Nature, Vol. 348, Issue 6303, pp. 681-2, 1991 (PubMed).

Harris: "p53: at the crossroads of molecular carcinogenesis and risk assessment." in: Science (New York, N.Y.), Vol. 262, Issue 5142, pp. 1980-1, 1994 (PubMed).

Levine: "p53, the cellular gatekeeper for growth and division." in: Cell, Vol. 88, Issue 3, pp. 323-31, 1997 (PubMed).

Lakin, Jackson: "Regulation of p53 in response to DNA damage." in: Oncogene, Vol. 18, Issue 53, pp. 7644-55, 2000 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Tumor Protein P53 (TP53)", type "Antibodies"
Hosts Rabbit (473), Mouse (290), Sheep (4), Escherichia Coli (E. coli) (1), Rat (1), Recombinant (1)
Reactivities Human (705), Mouse (Murine) (176), Rat (Rattus) (145), Cow (Bovine) (69), Dog (Canine) (49), Horse (Equine) (48), Pig (Porcine) (43), Sheep (Ovine) (41), Monkey (40), Guinea Pig (10), Primate (8), Chicken (7), Rabbit (7), Cat (Feline) (5), Hamster (5), Mammalian (2), Xenopus laevis (2), Bird (Avian) (1)
Applications Western Blotting (WB) (542), ELISA (232), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (215), Immunofluorescence (IF) (199), Immunoprecipitation (IP) (162), Immunohistochemistry (IHC) (146), Flow Cytometry (FACS) (93), Immunocytochemistry (ICC) (58), Immunohistochemistry (Frozen Sections) (IHC (fro)) (50), Immunohistochemistry (Fixed) (IHC (fx)) (22), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (10), Dot Blot (Dot) (9), Chromatin Immunoprecipitation (ChiP) (8), Gel Shift (GS) (5), Radioimmunoassay (RIA) (4), Immunoelectron Microscopy (IEM) (2), Affinity Purification (AP) (1), Cellular Assay (CA) (1), Dot Blot (DB) (1), ELISA (Capture) (1), ELISA (Detection) (1), Immunoassay (IA) (1), Immunostaining (ISt) (1), Transfected cell culture (TCC) (1)
Conjugates FITC (17), Alexa Fluor 350 (15), Alexa Fluor 488 (15), Alexa Fluor 555 (15), Alexa Fluor 647 (15), Biotin (13), PE,Cy5.5 (13), HRP (4), PE (3), RPE (3), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE-Cy3 (2), PE-Cy5 (2), PE-Cy5.5 (2), PE-Cy7 (2), Un-conjugated (2)
Epitopes AA 20-25 (20), N-Term (18), pSer37 (17), pSer46 (16), C-Term (15), pSer15 (15), pSer315 (15), pSer33 (14), pThr18 (14), AA 1-393 (13), pSer20 (13), pSer6 (13), pSer392 (12), pSer9 (11), pSer376 (7), pThr55 (7), Ser37 (5), Ser46 (5), pThr81 (5), AA 14-389 (4), AA 371-380 (4), Ser315 (4), AA 10-16 (3), AA 26-35 (3), AA 32-79 (3), AA 35-45 (3), AA 56-65 (3), pSer378 (3), AA 16-25 (2), AA 16-30 (2), AA 181-190 (2), AA 213-217 (2), AA 306-393 (2), AA 378-393 (2), AA 46-53 (2), AA 46-55 (2), AA 88-109 (2), Ser15 (2), Ser33 (2), Ser6 (2), Thr18 (2), Thr55 (2), acLys317 (2), acLys379 (2), acLys382 (2), acLys386 (2), AA 1-12 (1), AA 14-289 (1), AA 15-40 (1), AA 156-214 (1), AA 361-382 (1), AA 367-381 (1), AA 37-45 (1), AA 37-5 (1), AA 383-393 (1), Internal Region (1), Mutant (1), Mutant,AA 213-217 (1), Ser15,Phosphospecific (1), Ser20 (1), Ser392 (1), Wild type,Mutant (1), Wild type,Mutant,AA 32-79 (1), Wild type,Mutant,AA 370-378 (1), Wildtype (1), acLys373 (1), pSer299 (1), pSer332 (1), pSer366 (1), pSer371 (1), pSer392,AA 378-393 (1), pSer63 (1), pSer95 (1), pThr387 (1), pTyr757 (1), phospho (1)