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Details for Product No. ABIN650197

p70-S6 (N-Term) antibody

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Antigen
Epitope
»Alternatives N-Term
Reactivity
»Alternatives Human, Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Monoclonal
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)
Pubmed 4 references available
Catalog no. ABIN650197
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen A synthetic peptide corresponding to residues in N-terminus of human p70 S6 kinase was used as immunogen. This antibody detects both alpha I and alpha II isoforms.
Specificity A synthetic peptide corresponding to residues in N-terminus of human p70 S6 kinase was used as immunogen. This antibody detects both alpha I and alpha II isoforms.
Background P70 S6 Kinase is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1). p70 S6 kinase phosphorylates specifically ribosomal protein S6. Activation of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (2). Activation occurs via phosphorylation at Ser411, Thr421 and Ser424 within the pseudosubstrate region (3, 4). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function (2).
Synonyms: RPS6KB1, STK14A, Ribosomal protein S6 kinase beta-1, 70 kDa ribosomal protein S6 kinase 1,p70 S6 kinase alpha
Molecular Weight 70 kDA
Gene ID 6198
UniProt P23443
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Chromatin, Neurology
Application Notes The suggested dilution is: WB: ~~ 1:5000~10000 IP: ~~ 1:80 FC: ~~ 1:20 IHC: ~~ 1:100~250
Comment

Background: p70 S6 Kinase is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1). p70 S6 kinase phosphorylates specifically ribosomal protein S6. Activation of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (2). Activation occurs via phosphorylation at Ser411, Thr421 and Ser424 within the pseudosubstrate region (3, 4). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function (2).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment p70-S6 Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Pullen, Thomas: "The modular phosphorylation and activation of p70s6k." in: FEBS letters, Vol. 410, Issue 1, pp. 78-82, 1997 (PubMed).

Pearson, Thomas: "Regulation of p70s6k/p85s6k and its role in the cell cycle." in: Progress in cell cycle research, Vol. 1, pp. 21-32, 1998 (PubMed).

Dufner, Thomas: "Ribosomal S6 kinase signaling and the control of translation." in: Experimental cell research, Vol. 253, Issue 1, pp. 100-9, 1999 (PubMed).

Le, Hittelman, Liu et al.: "Paclitaxel induces inactivation of p70 S6 kinase and phosphorylation of Thr421 and Ser424 via multiple signaling pathways in mitosis." in: Oncogene, Vol. 22, Issue 4, pp. 484-97, 2003 (PubMed).

Alternatives for antigen "p70-S6", type "Antibodies"
Hosts (1)
Reactivities (1)
Applications (1), (1)
Epitopes (1), (1)
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