p70-S6 (N-Term) antibody

Antigen: p70-S6

Binding Site: N-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650197

Additional Information

Gene ID: 6198

UniProt: P23443

Immunogen: A synthetic peptide corresponding to residues in N-terminus of human p70 S6 kinase was used as immunogen. This antibody detects both alpha I and alpha II isoforms.

Description: Other names: RPS6KB1, STK14A, Ribosomal protein S6 kinase beta-1, 70 kDa ribosomal protein S6 kinase 1,p70 S6 kinase alpha

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in N-terminus of human p70 S6 kinase was used as immunogen. This antibody detects both alpha I and alpha II isoforms.

Molecular Weight: 70 kDA

Comments:

Background: p70 S6 Kinase is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1). p70 S6 kinase phosphorylates specifically ribosomal protein S6. Activation of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (2). Activation occurs via phosphorylation at Ser411, Thr421 and Ser424 within the pseudosubstrate region (3, 4). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function (2).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:5000~10000 IP: ~~ 1:80 FC: ~~ 1:20 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: p70-S6 Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Chromatin, Neurology

Restrictions: For Research Use only

Publications

Product: Pullen, Thomas: "The modular phosphorylation and activation of p70s6k." in: FEBS letters, Vol. 410, Issue 1, pp. 78-82, 1997 (PubMed).

Pearson, Thomas: "Regulation of p70s6k/p85s6k and its role in the cell cycle." in: Progress in cell cycle research, Vol. 1, pp. 21-32, 1998 (PubMed).

Dufner, Thomas: "Ribosomal S6 kinase signaling and the control of translation." in: Experimental cell research, Vol. 253, Issue 1, pp. 100-9, 1999 (PubMed).

Le, Hittelman, Liu et al.: "Paclitaxel induces inactivation of p70 S6 kinase and phosphorylation of Thr421 and Ser424 via multiple signaling pathways in mitosis." in: Oncogene, Vol. 22, Issue 4, pp. 484-97, 2003 (PubMed).