|+1 404 474 4654|
|+1 888 205 9894 (TF)|
p70-S6 (N-Term) antibody
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)
|4 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||A synthetic peptide corresponding to residues in N-terminus of human p70 S6 kinase was used as immunogen. This antibody detects both alpha I and alpha II isoforms.|
|Description||Other names: RPS6KB1, STK14A, Ribosomal protein S6 kinase beta-1, 70 kDa ribosomal protein S6 kinase 1,p70 S6 kinase alpha|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues in N-terminus of human p70 S6 kinase was used as immunogen. This antibody detects both alpha I and alpha II isoforms.|
|Molecular Weight||70 kDA|
Background: p70 S6 Kinase is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1). p70 S6 kinase phosphorylates specifically ribosomal protein S6. Activation of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (2). Activation occurs via phosphorylation at Ser411, Thr421 and Ser424 within the pseudosubstrate region (3, 4). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function (2).
|Application Notes||The suggested dilution is: WB: ~~ 1:5000~10000 IP: ~~ 1:80 FC: ~~ 1:20 IHC: ~~ 1:100~250|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||p70-S6 Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Chromatin, Neurology|
|Restrictions||For Research Use only|
Pullen, Thomas: "The modular phosphorylation and activation of p70s6k." in: FEBS letters, Vol. 410, Issue 1, pp. 78-82, 1997 (PubMed).
Pearson, Thomas: "Regulation of p70s6k/p85s6k and its role in the cell cycle." in: Progress in cell cycle research, Vol. 1, pp. 21-32, 1998 (PubMed).
Dufner, Thomas: "Ribosomal S6 kinase signaling and the control of translation." in: Experimental cell research, Vol. 253, Issue 1, pp. 100-9, 1999 (PubMed).
Le, Hittelman, Liu et al.: "Paclitaxel induces inactivation of p70 S6 kinase and phosphorylation of Thr421 and Ser424 via multiple signaling pathways in mitosis." in: Oncogene, Vol. 22, Issue 4, pp. 484-97, 2003 (PubMed).