p70-S6 (pThr421, pSer424) antibody

Antigen: p70-S6

Binding Site: pThr421, pSer424

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650199

Additional Information

Gene ID: 6198

UniProt: P23443

Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr421 and Ser424 of human p70 S6 Kinase alpha was used as immunogen. The antibody only detects p70-S6K phosphorylated on Threonine 421 and Serine 424.

Description: Other names: RPS6KB1, STK14A, Ribosomal protein S6 kinase beta-1, 70 kDa ribosomal protein S6 kinase 1,p70 S6 kinase alpha

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic phospho-peptide corresponding to residues surrounding Thr421 and Ser424 of human p70 S6 Kinase alpha was used as immunogen. The antibody only detects p70-S6K phosphorylated on Threonine 421 and Serine 424.

Molecular Weight: 70 kDA

Comments:

Background: p70 S6 Kinase is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1). p70 S6 kinase phosphorylates specifically ribosomal protein S6. Activation of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (2). Activation occurs via phosphorylation at Ser411, Thr421 and Ser424 within the pseudosubstrate region (3, 4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:2500 IP: ~~ 1:20

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: p70-S6 Antibody Phospho-pT421/pS424 can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Chromatin, Neurology

Restrictions: For Research Use only

Publications

Product: Pullen, Thomas: "The modular phosphorylation and activation of p70s6k." in: FEBS letters, Vol. 410, Issue 1, pp. 78-82, 1997 (PubMed).

Pearson, Thomas: "Regulation of p70s6k/p85s6k and its role in the cell cycle." in: Progress in cell cycle research, Vol. 1, pp. 21-32, 1998 (PubMed).

Dufner, Thomas: "Ribosomal S6 kinase signaling and the control of translation." in: Experimental cell research, Vol. 253, Issue 1, pp. 100-9, 1999 (PubMed).

Le, Hittelman, Liu et al.: "Paclitaxel induces inactivation of p70 S6 kinase and phosphorylation of Thr421 and Ser424 via multiple signaling pathways in mitosis." in: Oncogene, Vol. 22, Issue 4, pp. 484-97, 2003 (PubMed).