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p70-S6 (pThr421), (pSer424) antibody

Details for Product No. ABIN650199
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Antigen
Epitope
pThr421, pSer424
(1)
Reactivity
Human
(1), (1)
Host
Rabbit
(1)
Clonality
Monoclonal
Application
Western Blotting (WB)
(1), (1)
Pubmed 4 references available
Quantity 100 µL
Options
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Availability Will be delivered in 2 to 3 Business Days
Catalog No. ABIN650199
507.14 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Thr421 and Ser424 of human p70 S6 Kinase alpha was used as immunogen. The antibody only detects p70-S6K phosphorylated on Threonine 421 and Serine 424.
Specificity A synthetic phospho-peptide corresponding to residues surrounding Thr421 and Ser424 of human p70 S6 Kinase alpha was used as immunogen. The antibody only detects p70-S6K phosphorylated on Threonine 421 and Serine 424.
Background P70 S6 Kinase is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1). p70 S6 kinase phosphorylates specifically ribosomal protein S6. Activation of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (2). Activation occurs via phosphorylation at Ser411, Thr421 and Ser424 within the pseudosubstrate region (3, 4).
Synonyms: RPS6KB1, STK14A, Ribosomal protein S6 kinase beta-1, 70 kDa ribosomal protein S6 kinase 1,p70 S6 kinase alpha
Molecular Weight 70 kDA
Gene ID 6198
UniProt P23443
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Chromatin, Neurology
Application Notes The suggested dilution is: WB: = 1:2500
Comment

Background: p70 S6 Kinase is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1). p70 S6 kinase phosphorylates specifically ribosomal protein S6. Activation of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (2). Activation occurs via phosphorylation at Ser411, Thr421 and Ser424 within the pseudosubstrate region (3, 4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment p70-S6 Antibody Phospho-pT421/pS424 can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Pullen, Thomas: "The modular phosphorylation and activation of p70s6k." in: FEBS letters, Vol. 410, Issue 1, pp. 78-82, 1997 (PubMed).

Pearson, Thomas: "Regulation of p70s6k/p85s6k and its role in the cell cycle." in: Progress in cell cycle research, Vol. 1, pp. 21-32, 1998 (PubMed).

Dufner, Thomas: "Ribosomal S6 kinase signaling and the control of translation." in: Experimental cell research, Vol. 253, Issue 1, pp. 100-9, 1999 (PubMed).

Le, Hittelman, Liu et al.: "Paclitaxel induces inactivation of p70 S6 kinase and phosphorylation of Thr421 and Ser424 via multiple signaling pathways in mitosis." in: Oncogene, Vol. 22, Issue 4, pp. 484-97, 2003 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN650199
507.14 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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