rho GTPase Activating Protein 24 (ARHGAP24) antibody

Antigen: rho GTPase Activating Protein 24 (ARHGAP24)

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650200

Additional Information

Alternative name: p73

Gene ID: 7161

UniProt: O15350

Immunogen: A synthetic peptide corresponding to residues near the transactivation domain of human p73 was used as immunogen. p73 has a predicted molecular weight of 73 k

Description: Other names: TP73, P73, Tumor protein p73, p53-like transcription factor,p53-related protein

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the transactivation domain of human p73 was used as immunogen. p73 has a predicted molecular weight of 73 kDa but is detected at 63kDa.

Molecular Weight: 73 kDA

Comments:

Background: p73 is a transcription factor that shares a significant amino acid identity with p53 and p63 (1). p73 and p63 can both activate p53-responsive genes and induce apoptosis upon over- expression (2) Unlike p53, p73 is not induced by DNA damage and is not a target for inactivation by viral oncoproteins (3). Similar to p63, p73 has an additional coding region in the C-terminus (not found in p53) that can undergo complex alternative splicing giving rise to 3 splice forms (α,, β,, and γ,) (4). Activation of p73 requires the presence of functional c-Abl. It has also be found that mutant p53 can bind to and inactivate p73 (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000 IP: ~~ 1:50 FC: ~~ 1:60 ICC: ~~ 1:150 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: p73 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Apoptosis/Necrosis, Translation Factors, Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Kaghad, Bonnet, Yang et al.: "Monoallelically expressed gene related to p53 at 1p36, a region frequently deleted in neuroblastoma and other human cancers." in: Cell, Vol. 90, Issue 4, pp. 809-19, 1997 (PubMed).

Yang, Kaghad, Wang et al.: "p63, a p53 homolog at 3q27-29, encodes multiple products with transactivating, death-inducing, and dominant-negative activities." in: Molecular cell, Vol. 2, Issue 3, pp. 305-16, 1998 (PubMed).