Nibrin (NBN) (pSer432) antibody

Antigen: Nibrin (NBN)

Synonyms: ATV, NBS, P95, NBS1, AT-V1, AT-V2, FLJ10155, MGC87362, Nbs1, NBN, DKFZp469A0911

Binding Site: pSer432

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN650201

Additional Information

Alternative name: p95 NBS1

Gene ID: 4683

UniProt: O60934

Immunogen: A phospho-specific peptide corresponding to residues surrounding serine 432 of human p95/NSB1 was used as an immunogen. This antibody detects p95/NSB1 that is phosphorylated on serine 432.

Characteristics: Rabbit Monoclonal Antibody

Specificity: A phospho-specific peptide corresponding to residues surrounding serine 432 of human p95/NSB1 was used as an immunogen. This antibody detects p95/NSB1 that is phosphorylated on serine 432.

Molecular Weight: 95 kDA

Comments:

Background: Nijmegen breakage syndrome (NBS) is characterized by extreme radiation sensitivity, chromosomal instability and cancer. p95/NBS1 is specifically phosphorylated in response to gamma-radiation, ultraviolet light and exposure to hydroxyurea. Phosphorylation of p95/NBS1 mediated by gamma-radiation, but not that induced by hydroxyurea or ultraviolet light, was markedly reduced in ATM cells. Phosphorylation of p95/NBS1 by ATM is critical for certain responses of human cells to DNA damage (1). p95/NBS1 is part of a protein complex that is involved in responses to DNA double-strand breaks. Observations link ATM and p95/NBS1 in a common signaling pathway and provide an explanation for phenotypic similarities in NBS and ATM diseases (2). Double-strand breaks occur during DNA replication and are also induced by ionizing radiation. NBS1 is essential for homologous recombination-mediated repair in higher vertebrate cells and the disruption of p95/NBS1 reduces gene conversion and sister chromatid exchanges (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: p95 NBS1 Antibody Phospho (pS432) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Lim, Kim, Xu et al.: "ATM phosphorylates p95/nbs1 in an S-phase checkpoint pathway." in: Nature, Vol. 404, Issue 6778, pp. 613-7, 2000 (PubMed).

Wang: "Cancer. New link in a web of human genes." in: Nature, Vol. 405, Issue 6785, pp. 404-5, 2000 (PubMed).

Tauchi, Kobayashi, Morishima et al.: "Nbs1 is essential for DNA repair by homologous recombination in higher vertebrate cells." in: Nature, Vol. 420, Issue 6911, pp. 93-8, 2002 (PubMed).