Nibrin (NBN) (pSer432) antibody

Details for Product No. ABIN650201
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Antigen
Synonyms NBN, AT-V1, AT-V2, ATV, NBS, NBS1, P95, Nbs1, im:6911679, zgc:194152
Epitope
pSer432
(48), (20), (8), (8), (7), (6), (6), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(221), (81), (55), (24), (24), (24)
Host
Rabbit
(178), (47)
Clonality
Monoclonal
Conjugate
Un-conjugated
(7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(183), (76), (46), (40), (35), (26), (24), (22), (12), (2), (2), (1)
Pubmed 3 references available
Quantity 100 µL
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Catalog No. ABIN650201
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Immunogen A phospho-specific peptide corresponding to residues surrounding serine 432 of human p95/NSB1 was used as an immunogen. This antibody detects p95/NSB1 that is phosphorylated on serine 432.
Specificity A phospho-specific peptide corresponding to residues surrounding serine 432 of human p95/NSB1 was used as an immunogen. This antibody detects p95/NSB1 that is phosphorylated on serine 432.
Alternative Name p95 NBS1
Background Nijmegen breakage syndrome (NBS) is characterized by extreme radiation sensitivity, chromosomal instability and cancer. p95/NBS1 is specifically phosphorylated in response to gamma-radiation, ultraviolet light and exposure to hydroxyurea. Phosphorylation of p95/NBS1 mediated by gamma-radiation, but not that induced by hydroxyurea or ultraviolet light, was markedly reduced in ATM cells. Phosphorylation of p95/NBS1 by ATM is critical for certain responses of human cells to DNA damage (1). p95/NBS1 is part of a protein complex that is involved in responses to DNA double-strand breaks. Observations link ATM and p95/NBS1 in a common signaling pathway and provide an explanation for phenotypic similarities in NBS and ATM diseases (2). Double-strand breaks occur during DNA replication and are also induced by ionizing radiation. NBS1 is essential for homologous recombination-mediated repair in higher vertebrate cells and the disruption of p95/NBS1 reduces gene conversion and sister chromatid exchanges (3).
Molecular Weight 95 kDA
Gene ID 4683
UniProt O60934
Research Area Phospho-specific antibodies, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure
Application Notes IHC: = 1:100-250, WB: = 1:500
Comment

Background: Nijmegen breakage syndrome (NBS) is characterized by extreme radiation sensitivity, chromosomal instability and cancer. p95/NBS1 is specifically phosphorylated in response to gamma-radiation, ultraviolet light and exposure to hydroxyurea. Phosphorylation of p95/NBS1 mediated by gamma-radiation, but not that induced by hydroxyurea or ultraviolet light, was markedly reduced in ATM cells. Phosphorylation of p95/NBS1 by ATM is critical for certain responses of human cells to DNA damage (1). p95/NBS1 is part of a protein complex that is involved in responses to DNA double-strand breaks. Observations link ATM and p95/NBS1 in a common signaling pathway and provide an explanation for phenotypic similarities in NBS and ATM diseases (2). Double-strand breaks occur during DNA replication and are also induced by ionizing radiation. NBS1 is essential for homologous recombination-mediated repair in higher vertebrate cells and the disruption of p95/NBS1 reduces gene conversion and sister chromatid exchanges (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment p95 NBS1 Antibody Phospho (pS432) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Lim, Kim, Xu et al.: "ATM phosphorylates p95/nbs1 in an S-phase checkpoint pathway." in: Nature, Vol. 404, Issue 6778, pp. 613-7, 2000 (PubMed).

Wang: "Cancer. New link in a web of human genes." in: Nature, Vol. 405, Issue 6785, pp. 404-5, 2000 (PubMed).

Tauchi, Kobayashi, Morishima et al.: "Nbs1 is essential for DNA repair by homologous recombination in higher vertebrate cells." in: Nature, Vol. 420, Issue 6911, pp. 93-8, 2002 (PubMed).

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