|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Nibrin (NBN) (pSer432) antibody
|Synonyms||ATV, NBS, P95, NBS1, AT-V1, AT-V2, FLJ10155, MGC87362, Nbs1, NBN, DKFZp469A0911|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
|3 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||p95 NBS1|
|Immunogen||A phospho-specific peptide corresponding to residues surrounding serine 432 of human p95/NSB1 was used as an immunogen. This antibody detects p95/NSB1 that is phosphorylated on serine 432.|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A phospho-specific peptide corresponding to residues surrounding serine 432 of human p95/NSB1 was used as an immunogen. This antibody detects p95/NSB1 that is phosphorylated on serine 432.|
|Molecular Weight||95 kDA|
Background: Nijmegen breakage syndrome (NBS) is characterized by extreme radiation sensitivity, chromosomal instability and cancer. p95/NBS1 is specifically phosphorylated in response to gamma-radiation, ultraviolet light and exposure to hydroxyurea. Phosphorylation of p95/NBS1 mediated by gamma-radiation, but not that induced by hydroxyurea or ultraviolet light, was markedly reduced in ATM cells. Phosphorylation of p95/NBS1 by ATM is critical for certain responses of human cells to DNA damage (1). p95/NBS1 is part of a protein complex that is involved in responses to DNA double-strand breaks. Observations link ATM and p95/NBS1 in a common signaling pathway and provide an explanation for phenotypic similarities in NBS and ATM diseases (2). Double-strand breaks occur during DNA replication and are also induced by ionizing radiation. NBS1 is essential for homologous recombination-mediated repair in higher vertebrate cells and the disruption of p95/NBS1 reduces gene conversion and sister chromatid exchanges (3).
|Application Notes||The suggested dilution is: WB: ~~ 1:500 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||p95 NBS1 Antibody Phospho (pS432) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Phospho-specific antibodies, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Lim, Kim, Xu et al.: "ATM phosphorylates p95/nbs1 in an S-phase checkpoint pathway." in: Nature, Vol. 404, Issue 6778, pp. 613-7, 2000 (PubMed).
Wang: "Cancer. New link in a web of human genes." in: Nature, Vol. 405, Issue 6785, pp. 404-5, 2000 (PubMed).
Tauchi, Kobayashi, Morishima et al.: "Nbs1 is essential for DNA repair by homologous recombination in higher vertebrate cells." in: Nature, Vol. 420, Issue 6911, pp. 93-8, 2002 (PubMed).
|Hosts||Rabbit (108), Mouse (10)|
|Reactivities||Human (117), Rat (Rattus) (57), Mouse (Murine) (51), Chicken (37), Cow (Bovine) (37), Dog (Canine) (37), Horse (Equine) (36), Cat (Feline) (1)|
|Applications||Western Blotting (WB) (74), Immunofluorescence (IF) (50), ELISA (39), Immunoprecipitation (IP) (21), Immunohistochemistry (IHC) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunocytochemistry (ICC) (5), Immunoelectron Microscopy (IEM) (2)|
|Conjugates||Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Biotin (3), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2)|
|Epitopes||pSer343 (22), C-Term (9), Internal Region (3), N-Term (2), Ser343 (2), AA 119-134 (1), AA 350-400 (1), AA 395-742 (1), AA 420-774 (1), AA 425-475 (1), AA 625-675 (1), AA 701-751 (1), AA 704-754 (1), pSer278 (1), pTyr99 (1)|