P21-Activated Kinase 2 (PAK2) (pSer20) antibody

Antigen: P21-Activated Kinase 2 (PAK2)

Synonyms: PAK65, PAKgamma, gamma-PAK, PAK-2, AI836325, KIAA4182, mKIAA4182, D16Ertd269e, A130002K10Rik, pak2, cb422, kinase, zgc:91798, wu:fb71h01, si:dz198m22.1, PAK2

Binding Site: pSer20

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN650210

Additional Information

Alternative name: PAK2

Gene ID: 5062

UniProt: Q13177

Immunogen: A phospho-specific peptide corresponding to residues surrounding Serine 20 of human PAK2 was used as an immunogen.

Description: Other names: PAK2, PAK-2p34, C-t-PAK2

Characteristics: Rabbit Monoclonal Antibody

Specificity: A phospho-specific peptide corresponding to residues surrounding Serine 20 of human PAK2 was used as an immunogen.

Molecular Weight: 61 kDA

Comments:

Background: p21-activated kinase 2 (PAK2) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). All six PAKs play an important role in diverse cellular processes, including cytoskeletal dynamics, growth/apoptotic signal transduction through MAP kinases, and regulation of transcription factors (4). PAK2 is unique among the PAK isoforms because it is also activated through proteolytic cleavage by caspases or caspase-like proteases (5). Full-length PAK-2 is localized in the cytoplasm, whereas the proteolytic PAK-2p34 fragment translocates to the nucleus. Activation of full-length PAK-2 stimulates cell survival, and proteolytic activation of PAK-2p34 is involved in programmed cell death (6). Phosphorylation within the Nck (Serine 20) and Pak-interacting guanine nucleotide exchange factor (Serine 192 and Serine 197) binding sites blocks the interactions of Pak 2 with certain proteins (7).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:10000~50000 ICC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: PAK2 Antibody Phospho (pS20) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Protein Modifications, Signaling, Cell Structure, Ubiquitin-related antibodies, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Cell Cycle, Chromatin

Restrictions: For Research Use only

Publications

Product: Gnesutta, Qu, Minden: "The serine/threonine kinase PAK4 prevents caspase activation and protects cells from apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 17, pp. 14414-9, 2001 (PubMed).

Zhan, Ge, Ohira et al.: "p21-activated kinase 2 in neutrophils can be regulated by phosphorylation at multiple sites and by a variety of protein phosphatases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 7, pp. 3785-93, 2003 (PubMed).