You are viewing an incomplete version of our website. Please click to reload the website as full version.

PAK2 antibody (P21-Activated Kinase 2) (pSer20)

Details for Product anti-PAK2 Antibody No. ABIN650210, Supplier: Login to see New
New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Alternatives
Epitope
pSer20
(45), (24), (14), (9), (7), (6), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(179), (77), (76), (6), (5), (2), (1)
Host
Rabbit
(164), (15)
Clonality
Monoclonal
Conjugate
This PAK2 antibody is un-conjugated
(4), (4), (4), (4), (4), (4), (3)
Application
Western Blotting (WB)
(156), (98), (73), (22), (16), (5), (5), (4), (3), (1), (1)
Pubmed 2 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 100 μL
Options
Shipping to United States ( )
Availability
Immunogen A phospho-specific peptide corresponding to residues surrounding Serine 20 of human PAK2 was used as an immunogen.
Specificity A phospho-specific peptide corresponding to residues surrounding Serine 20 of human PAK2 was used as an immunogen.
Alternative Name PAK2 (PAK2 Antibody Abstract)
Background P21-activated kinase 2 (PAK2) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). All six PAKs play an important role in diverse cellular processes, including cytoskeletal dynamics, growth/apoptotic signal transduction through MAP kinases, and regulation of transcription factors (4). PAK2 is unique among the PAK isoforms because it is also activated through proteolytic cleavage by caspases or caspase-like proteases (5). Full-length PAK-2 is localized in the cytoplasm, whereas the proteolytic PAK-2p34 fragment translocates to the nucleus. Activation of full-length PAK-2 stimulates cell survival, and proteolytic activation of PAK-2p34 is involved in programmed cell death (6). Phosphorylation within the Nck (Serine 20) and Pak-interacting guanine nucleotide exchange factor (Serine 192 and Serine 197) binding sites blocks the interactions of Pak 2 with certain proteins (7).
Synonyms: PAK2, PAK-2p34, C-t-PAK2
Molecular Weight 61 kDA
Gene ID 5062
UniProt Q13177
Research Area Protein Modifications, Signaling, Cell Structure, Ubiquitin-related antibodies, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Cell Cycle, Chromatin
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway
Application Notes The suggested dilution is: WB: = 1:10000-50000
Comment

Background: p21-activated kinase 2 (PAK2) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). All six PAKs play an important role in diverse cellular processes, including cytoskeletal dynamics, growth/apoptotic signal transduction through MAP kinases, and regulation of transcription factors (4). PAK2 is unique among the PAK isoforms because it is also activated through proteolytic cleavage by caspases or caspase-like proteases (5). Full-length PAK-2 is localized in the cytoplasm, whereas the proteolytic PAK-2p34 fragment translocates to the nucleus. Activation of full-length PAK-2 stimulates cell survival, and proteolytic activation of PAK-2p34 is involved in programmed cell death (6). Phosphorylation within the Nck (Serine 20) and Pak-interacting guanine nucleotide exchange factor (Serine 192 and Serine 197) binding sites blocks the interactions of Pak 2 with certain proteins (7).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment PAK2 Antibody Phospho (pS20) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Zhan, Ge, Ohira et al.: "p21-activated kinase 2 in neutrophils can be regulated by phosphorylation at multiple sites and by a variety of protein phosphatases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 7, pp. 3785-93, 2003 (PubMed).

Gnesutta, Qu, Minden: "The serine/threonine kinase PAK4 prevents caspase activation and protects cells from apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 17, pp. 14414-9, 2001 (PubMed).

Catalog No. ABIN650210

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)