P21-Activated Kinase 2 (PAK2) (pSer20) antibody

Details for Product No. ABIN650210
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Antigen
Synonyms pak2, cb422, kinase, zgc:91798, wu:fb71h01, si:dz198m22.1, pak65, pakgamma, PAK65, PAKgamma, 5330420P17Rik, A130002K10Rik, AI836325, D16Ertd269e, PAK-2, mKIAA4182, gamma-PAK, PAKI, Pak3
Epitope
pSer20
(27), (19), (18), (16), (14), (8), (5), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(177), (117), (116), (37), (36), (12), (7), (1), (1)
Host
Rabbit
(165), (13)
Clonality
Monoclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application
Western Blotting (WB)
(139), (98), (64), (30), (28), (9), (4), (4), (2), (1)
Pubmed 2 references available
Quantity 100 µL
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Catalog No. ABIN650210
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Immunogen A phospho-specific peptide corresponding to residues surrounding Serine 20 of human PAK2 was used as an immunogen.
Specificity A phospho-specific peptide corresponding to residues surrounding Serine 20 of human PAK2 was used as an immunogen.
Alternative Name PAK2
Background P21-activated kinase 2 (PAK2) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). All six PAKs play an important role in diverse cellular processes, including cytoskeletal dynamics, growth/apoptotic signal transduction through MAP kinases, and regulation of transcription factors (4). PAK2 is unique among the PAK isoforms because it is also activated through proteolytic cleavage by caspases or caspase-like proteases (5). Full-length PAK-2 is localized in the cytoplasm, whereas the proteolytic PAK-2p34 fragment translocates to the nucleus. Activation of full-length PAK-2 stimulates cell survival, and proteolytic activation of PAK-2p34 is involved in programmed cell death (6). Phosphorylation within the Nck (Serine 20) and Pak-interacting guanine nucleotide exchange factor (Serine 192 and Serine 197) binding sites blocks the interactions of Pak 2 with certain proteins (7).
Synonyms: PAK2, PAK-2p34, C-t-PAK2
Molecular Weight 61 kDA
Gene ID 5062
UniProt Q13177
Research Area Protein Modifications, Signaling, Cell Structure, Ubiquitin-related antibodies, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Cell Cycle, Chromatin
Application Notes The suggested dilution is: WB: = 1:10000-50000
Comment

Background: p21-activated kinase 2 (PAK2) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). All six PAKs play an important role in diverse cellular processes, including cytoskeletal dynamics, growth/apoptotic signal transduction through MAP kinases, and regulation of transcription factors (4). PAK2 is unique among the PAK isoforms because it is also activated through proteolytic cleavage by caspases or caspase-like proteases (5). Full-length PAK-2 is localized in the cytoplasm, whereas the proteolytic PAK-2p34 fragment translocates to the nucleus. Activation of full-length PAK-2 stimulates cell survival, and proteolytic activation of PAK-2p34 is involved in programmed cell death (6). Phosphorylation within the Nck (Serine 20) and Pak-interacting guanine nucleotide exchange factor (Serine 192 and Serine 197) binding sites blocks the interactions of Pak 2 with certain proteins (7).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment PAK2 Antibody Phospho (pS20) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Gnesutta, Qu, Minden: "The serine/threonine kinase PAK4 prevents caspase activation and protects cells from apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 17, pp. 14414-9, 2001 (PubMed).

Zhan, Ge, Ohira et al.: "p21-activated kinase 2 in neutrophils can be regulated by phosphorylation at multiple sites and by a variety of protein phosphatases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 7, pp. 3785-93, 2003 (PubMed).

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