PARP-1 (p25) (Cleaved) antibody

Antigen: PARP-1 (p25)

Binding Site: Cleaved

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650216

Additional Information

Gene ID: 142

UniProt: P09874

Immunogen: A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. The antibody only recognize p25 cleaved-form of PARP-1.

Description: Other names: PARP1, ADPRT, PPOL, Poly [ADP-ribose] polymerase 1, ADPRT,NAD(+) ADP-ribosyltransferase 1,Poly[ADP-ribose] synthetase 1

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. The antibody only recognize p25 cleaved-form of PARP-1.

Molecular Weight: 25 kDA

Comments:

Background: Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2,4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~5000 IP: ~~ 1:80 FC: ~~ 1:50 ICC: ~~ 1:250 IHC: ~~ 1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: PARP-1 Antibody (Cleaved p25) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Tewari, Quan, ORourke et al.: "Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase." in: Cell, Vol. 81, Issue 5, pp. 801-9, 1995 (PubMed).

Lazebnik, Kaufmann, Desnoyers et al.: "Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE." in: Nature, Vol. 371, Issue 6495, pp. 346-7, 1994 (PubMed).

Kaufmann, Desnoyers, Ottaviano et al.: "Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis." in: Cancer research, Vol. 53, Issue 17, pp. 3976-85, 1993 (PubMed).