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PARP-1 (p25) (cleaved) antibody

Details for Product No. ABIN650216
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Antigen
Epitope
cleaved
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Pubmed 3 references available
Quantity 100 µL
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Catalog No. ABIN650216
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Immunogen A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. The antibody only recognize p25 cleaved-form of PARP-1.
Specificity A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. The antibody only recognize p25 cleaved-form of PARP-1.
Background Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2,4).
Synonyms: PARP1, ADPRT, PPOL, Poly [ADP-ribose] polymerase 1, ADPRT,NAD(+) ADP-ribosyltransferase 1,Poly[ADP-ribose] synthetase 1
Molecular Weight 25 kDA
Gene ID 142
UniProt P09874
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-5000 , IHC: = 1:100
Comment

Background: Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2,4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment PARP-1 Antibody (Cleaved p25) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Tewari, Quan, ORourke et al.: "Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase." in: Cell, Vol. 81, Issue 5, pp. 801-9, 1995 (PubMed).

Lazebnik, Kaufmann, Desnoyers et al.: "Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE." in: Nature, Vol. 371, Issue 6495, pp. 346-7, 1994 (PubMed).

Kaufmann, Desnoyers, Ottaviano et al.: "Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis." in: Cancer research, Vol. 53, Issue 17, pp. 3976-85, 1993 (PubMed).

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