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PARP-1 (p116) antibody
Western Blotting (WB), Immunoprecipitation (IP)
|3 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. The antibody only recognizes full-length form (p116) of PARP-1.|
|Description||Other names: PARP1, ADPRT, PPOL, Poly [ADP-ribose] polymerase 1, ADPRT,NAD(+) ADP-ribosyltransferase 1,Poly[ADP-ribose] synthetase 1|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. The antibody only recognizes full-length form (p116) of PARP-1.|
|Molecular Weight||113 kDA|
Background: Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2,4).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000 IP: ~~ 1:80|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||PARP-1 Antibody (p116) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Tewari, Quan, ORourke et al.: "Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase." in: Cell, Vol. 81, Issue 5, pp. 801-9, 1995 (PubMed).
Lazebnik, Kaufmann, Desnoyers et al.: "Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE." in: Nature, Vol. 371, Issue 6495, pp. 346-7, 1994 (PubMed).
Kaufmann, Desnoyers, Ottaviano et al.: "Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis." in: Cancer research, Vol. 53, Issue 17, pp. 3976-85, 1993 (PubMed).