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Poly (ADP-Ribose) Polymerase 1 (PARP1) antibody

Details for Product No. ABIN650219
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Antigen
Synonyms
5830444G22Rik, AI893648, ARTD1, Adprp, Adprt1, C80510, PARP, PPOL, parp-1, sPARP-1, ADPRT, ADPRT 1, ADPRT1, PARP-1, pADPRT-1, Adprt, Parp-1, BEST:LD21673, CG17685, CG17696, CG17718, CG40411, D.PARP, D ... show more
5830444G22Rik, AI893648, ARTD1, Adprp, Adprt1, C80510, PARP, PPOL, parp-1, sPARP-1, ADPRT, ADPRT 1, ADPRT1, PARP-1, pADPRT-1, Adprt, Parp-1, BEST:LD21673, CG17685, CG17696, CG17718, CG40411, D.PARP, Dm.pARTa, Dmel\\CG40411, LD21673.3prime, PARP1, dPARP, parp, adprt1, padprt-1, ppol, ART7.1, ART1 show less
Reactivity
Human, Rat (Rattus)
(207), (43), (41), (23), (13), (2), (2), (2), (2), (1), (1), (1)
Host
Rabbit
(215), (28), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(13), (13), (12), (12), (12), (11), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(140), (115), (85), (46), (29), (23), (18), (16), (10), (9), (8), (1), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 100 µL
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Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Catalog No. ABIN650219
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A synthetic peptide corresponding to N-terminal residues of human PARP-1 was used as immunogen. The antibody should recognize both pro-form and p25 cleaved-form of PARP-1.
Specificity A synthetic peptide corresponding to N-terminal residues of human PARP-1 was used as immunogen. The antibody should recognize both pro-form and p25 cleaved-form of PARP-1.
Alternative Name PARP-1 (p116/p25)
Background Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2,4).
Synonyms: PARP1, ADPRT, PPOL, Poly [ADP-ribose] polymerase 1, ADPRT,NAD(+) ADP-ribosyltransferase 1,Poly[ADP-ribose] synthetase 1
Molecular Weight 116/25 kDA
Gene ID 142
UniProt P09874
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-2000 FACS: = 1:50 ICC: = 1:100 IHC: = 1:25
Comment

Background: Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2,4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment PARP-1 Antibody (p116/p25) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Tewari, Quan, ORourke et al.: "Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase." in: Cell, Vol. 81, Issue 5, pp. 801-9, 1995 (PubMed).

Kaufmann, Desnoyers, Ottaviano et al.: "Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis." in: Cancer research, Vol. 53, Issue 17, pp. 3976-85, 1993 (PubMed).

Hosts (215), (28), (1)
Reactivities (207), (43), (41), (23), (13), (2), (2), (2), (2), (1), (1), (1)
Applications (140), (115), (85), (46), (29), (23), (18), (16), (10), (9), (8), (1), (1), (1), (1), (1)
Conjugates (13), (13), (12), (12), (12), (11), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (35), (9), (9), (9), (9), (9), (9), (9), (9), (9), (9), (8), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN650219
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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